Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma

Autor:	Chopde Ct, Manish M. Wanjari, Sudhir N. Umathe, There Aw, Tajne Mr
Jazyk:	angličtina
Rok vydání:	2008
Předmět:	Chromatography diabetes Pharmaceutical Science Plasma Metformin chemistry.chemical_compound chemistry Pharmacokinetics medicine Acetonitrile metformin Ammonium acetate Retention time pharmacokinetics medicine.drug Research Paper
Zdroj:	Indian Journal of Pharmaceutical Sciences
ISSN:	1998-3743 0250-474X
Popis:	A simple reverse phase high-performance liquid chromatographic method has been developed for determining the concentration of metformin in rat plasma. The method employs C(18) column (300 mm x 2.4 mm i.d.), ammonium acetate (0.15 M) and acetonitrile (90:10; pH-5.5; 1.0 ml/min) as mobile phase and ultraviolet detection at 236 nm. Acetonitrile was used to simultaneously deproteinize rat plasma and extract metformin. The assay was linear in the concentration range of 0.33 mug-16.6 mug/ml with co-efficient of correlation 0.994. The retention time was 4.7 min. The method was found to be precise (% CV15%), accurate and suitable for pharmacokinetic study of orally administered metformin in rats.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94eac938b9acc700e94c4000ce0e1f82 http://europepmc.org/articles/PMC2792473 Zobrazit plný text záznamu Plný text ve formátu PDF