Expression of cspH upon nutrient up-shift in Salmonella enterica serovar Typhimurium
| Autor: | Iel Soo Bang, Hyeon Guk Kim, Jong Hak Choi, Gun Ick Bae, Bae Hoon Kim, Yong Keun Park, Sung Ho Bang |
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| Rok vydání: | 2004 |
| Předmět: |
Salmonella typhimurium
Cell division lac operon Biochemistry Microbiology DNA gyrase chemistry.chemical_compound Bacterial Proteins Factor For Inversion Stimulation Protein Genetics RNA Messenger Molecular Biology Gene Heat-Shock Proteins Messenger RNA biology Temperature Gene Expression Regulation Bacterial General Medicine biology.organism_classification Enterobacteriaceae Molecular biology Culture Media Cold Temperature chemistry DNA Gyrase Salmonella enterica DNA |
| Zdroj: | Archives of Microbiology. 182:37-43 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-004-0692-2 |
| Popis: | The gene cspH, which encodes one of the cold-shock proteins in Salmonella enterica serovar Typhimurium, has previously been reported to be induced during early exponential phase at 37 degrees C. In the present study, the expression of cspH upon nutrient up-shift at 37 degrees C was investigated and found to be affected by DNA gyrase and DNA-binding protein Fis. When cells at stationary phase were subcultured into a rich medium, the mRNA level of cspH increased dramatically prior to the first cell division. However, when the cells were treated with DNA gyrase inhibitors, cspH mRNA was not induced upon nutrient up-shift. The low level of DNA superhelical density at the cspH promoter in part affected the expression of cspH mRNA in vitro. In addition, a fis-deficient strain had a lower level of cspH mRNA than the wild-type upon nutrient up-shift. Finally, a cspH-lacZ construct, in which the putative binding region for Fis was deleted in the cspH promoter, expressed a low level of LacZ, in contrast to the native cspH-lacZ construct. |
| Databáze: | OpenAIRE |
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