Effects of transgenic expression of Brevibacterium linens methionine gamma lyase (MGL) on accumulation of Tylenchulus semipenetrans and key aminoacid contents in Carrizo citrange
| Autor: | Susan E. Ebeler, M. V. McKenry, Federico Martinelli, Elenor Castillo, Tom R. Buzo, Florence Zakharov-Negre, Abhaya M. Dandekar |
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| Přispěvatelé: | Castillo, Elenor, Martinelli, Federico, Zakharov-Negre, Florence, Ebeler, Susan E., Buzo, Tom R., McKenry, Michael V., Dandekar, Abhaya M. |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nematodes Plant Biology Plant Science Genetically modified crops 01 natural sciences Plant Roots chemistry.chemical_compound Methionine Methionine gamma lyase Brevibacterium Amino Acids Nematode Citrus sinensi biology Sulfur volatile food and beverages General Medicine Plants Plants Genetically Modified Tylenchulus semipenetrans Amino Acid Horticulture Carbon-Sulfur Lyases Biochemistry Carbon-Sulfur Lyase Plant Leave Citrus × sinensis Citrus sinensis Tylenchida Methionine gamma-lyase Sulfide Transgene Plant Biology & Botany Plant Disease Genetically Modified Sulfides Article 03 medical and health sciences Genetic Sulfur volatiles Genetics Animals Plant Diseases Animal fungi Plant Root biology.organism_classification Brain Disorders Plant Leaves 030104 developmental biology chemistry Glycine Biochemistry and Cell Biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant molecular biology, vol 95, iss 4-5 Plant Molecular Biology |
| Popis: | Key message Carrizo transgenic plants overexpressing methionine-gamma-lyase produced dimethyl sulfide. The transgenic plants displayed more resistance to nematode attacks (Tylenculus semipenetrans) and may represent an innovative strategy for nematode control. Abstract Tylenchulus semipenetrans is a nematode pest of many citrus varieties that causes extensive damage to commercial crops worldwide. Carrizo citrange vr. (Citrus sinensis L. Usb × Poncirus trifoliate L. Raf) plants overexpressing Brevibacterium linens methionine-gamma-lyase (BlMGL) produced the sulfur volatile compound dimethyl sulfide (DMS). The aim of this work was to determine if transgenic citrus plants expressing BlMGL showed increased tolerance to T. semipenetrans infestation and to determine the effect on the content of key amino acids. While transgenic lines emitted dimethyl sulfide from leaves and roots, no sulfur-containing volatiles were detectable in wild-type Carrizo in the same tissues. Significant changes detected some key amino acids from leaves of transgenic plants such as aspartate, lysine, glycine, leucine and threonine with no changes in the amounts of methionine and α-ketobutyrate. In roots only glycine showed significant changes across all transgenic lines in comparison to wild-type plants. Transgenic plants expressing BlMGL and emitting DMS had less T. semipenetrans aggregation and more biomass than infected WT control plants, indicating that they may represent an innovative management alternative to pesticide/nematicide-based remedies. Electronic supplementary material The online version of this article (doi:10.1007/s11103-017-0666-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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