Characterization of Fetal Cells from the Maternal Circulation by Microarray Gene Expression Analysis - Could the Extravillous Trophoblasts Be a Target for Future Cell-Based Non-Invasive Prenatal Diagnosis?
| Autor: | Rune Hoff Lauridsen, Berthold Huppertz, Marie Brinch, Niels Uldbjerg, Steen Kølvraa, Britta Christensen, Ripudaman Singh, Lotte Hatt, Kristine Møller |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Embryology medicine.medical_specialty Microarray Trophoblasts/physiology Prenatal diagnosis Andrology Pregnancy Prenatal Diagnosis/methods Microarray gene expression Prenatal Diagnosis Gene expression Humans Medicine Radiology Nuclear Medicine and imaging Maternal-Fetal Exchange Tissue Array Analysis/methods Fetal cells in maternal blood Regulation of gene expression Fetus Maternal-Fetal Exchange/physiology business.industry Obstetrics Gene Expression Regulation Developmental Obstetrics and Gynecology General Medicine Extravillous trophoblast medicine.disease Trophoblasts Chorionic Villi/physiology Tissue Array Analysis embryonic structures Pediatrics Perinatology and Child Health Maternal Serum Screening Tests/methods Female Chorionic Villi business Maternal Serum Screening Tests Cell based |
| Zdroj: | Hatt, L, Brinch, M, Singh, R, Møller, U K, Lauridsen, R H, Uldbjerg, N, Huppertz, B, Christensen, C B & Kølvraa, S 2014, ' Characterization of Fetal Cells from the Maternal Circulation by Microarray Gene Expression Analysis-Could the Extravillous Trophoblasts Be a Target for Future Cell-Based Non-Invasive Prenatal Diagnosis? ', Fetal Diagnosis and Therapy, vol. 35, pp. 218-227 . https://doi.org/10.1159/000356073 Hatt, L, Brinch, M, Singh, R, Møller, K, Lauridsen, R H, Uldbjerg, N, Huppertz, B, Christensen, B & Kølvraa, S 2014, ' Characterization of fetal cells from the maternal circulation by microarray gene expression analysis-Could the extravillous trophoblasts be a target for future cell-based non-invasive prenatal diagnosis? ', Fetal Diagnosis and Therapy, vol. 35, no. 3, pp. 218-227 . https://doi.org/10.1159/000356073 |
| ISSN: | 1421-9964 1015-3837 |
| DOI: | 10.1159/000356073 |
| Popis: | Introduction: Circulating fetal cells in maternal blood provide a tool for risk-free, non-invasive prenatal diagnosis. However, fetal cells in the maternal circulation are scarce, and to effectively isolate enough of them for reliable diagnostics, it is crucial to know which fetal cell type(s) should be targeted. Materials and Methods: Fetal cells were enriched from maternal blood by magnetic-activated cell sorting using the endothelial cell marker CD105 and identified by XY fluorescence in situ hybridization. Expression pattern was compared between fetal cells and maternal blood cells using stem cell microarray analysis. Results: 39 genes were identified as candidates for unique fetal cell markers. More than half of these are genes known to be expressed in the placenta, especially in extravillous trophoblasts (EVTs). Immunohistochemical staining of placental tissue confirmed CD105 staining in EVTs and 76% of fetal cells enriched by CD105 were found to be cytokeratin-positive. Discussion: The unique combination of mesodermal (CD105) and ectodermal (cytokeratin) markers in EVTs could be a potential marker set for cell enrichment of this cell type in maternal blood and could be the basis for future cell-based non-invasive prenatal diagnosis. |
| Databáze: | OpenAIRE |
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