Effect of microcystin on the expression of Nrf2 and its downstream antioxidant genes from Cristaria plicata
| Autor: | Chungen Wen, Wenxiu Liu, Shaoqing Jian, Guo-Ming Qian, Jielian Wu, Jie Dong, Baoqing Hu, Gang Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Unionidae
DNA Complementary Microcystins NF-E2-Related Factor 2 Health Toxicology and Mutagenesis Gene Expression Microcystin 010501 environmental sciences Aquatic Science Biology medicine.disease_cause 01 natural sciences Cristaria plicata Antioxidants law.invention 03 medical and health sciences law Complementary DNA medicine Animals Amino Acid Sequence Cloning Molecular Phylogeny 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences Gene knockdown Base Sequence Toxin biology.organism_classification Molecular biology Recombinant Proteins Enzyme chemistry Recombinant DNA Hepatopancreas Water Pollutants Chemical |
| Zdroj: | Aquatic Toxicology. 225:105526 |
| ISSN: | 0166-445X |
| DOI: | 10.1016/j.aquatox.2020.105526 |
| Popis: | Microcystin (MC) is a cyclic heptapeptide toxin. Nuclear factor erythocyte 2-related factor 2 (Nrf2) can enhance cellular survival by mediating phase 2 detoxification and antioxidant genes. In this study, CpNrf2 cDNA sequences were cloned from freshwater bivalve Cristaria plicata. The full-length CpNrf2 cDNA sequence was 4259 bp, and its homology was the highest with Mizuhopecten yessoensis, reaching 46%. CpNrf2 transcription levels were examined in all tested tissues, and the highest level was in hepatopancreas from C. plicata. The recombinant protein pET32-CpNrf2 was purified with the content of 1.375 mg/mL. The expression levels of CpNrf2 mRNA were raised in hepatopancreas after MC stimulation. After CpNrf2 knockdown, CpNrf2 mRNA levels were significantly down-regulated after 24 h. Compared with control group, the expression levels of ARE-driven enzymes (CpMnSOD, CpCuZnSOD, CpTRX, CpPrx, CpSe-GPx and Cpsigma-GST) were significantly increased, and those enzyme activities were also significantly up-regulated in MC-stimulated group. However, in CpNrf2-iRNA group, they were significantly down-regulated. The results revealed that Nrf2/ARE pathway is very crucial to protect molluscs from MC. |
| Databáze: | OpenAIRE |
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