Formation of purple hemocyanin upon addition of ethyleneglycol to Sepioteuthis lessoniana oxyhemocyanin
| Autor: | Masazo Kimura, Shinnichiro Suzuki, Osamu Yamauchi, Akitsugu Nakahara, Wasuke Mori |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
biology
medicine.medical_treatment Decapodiformes chemistry.chemical_element Active site Hemocyanin biology.organism_classification Biochemistry Copper Inorganic Chemistry Sepioteuthis Oxygen Crystallography Oxyhemocyanin chemistry Spectrophotometry Hemocyanins medicine biology.protein Animals Ethylene Glycols Oxidation-Reduction |
| Zdroj: | Journal of inorganic biochemistry. 12(2) |
| ISSN: | 0162-0134 |
| Popis: | We report the formation of purple hemocyanin upon addition of ethyleneglycol to a solution of Sepioteuthis lessoniana oxyhemocyanin in 0.05 M Tris-HCl buffer (pH 7.5). Spectroscopic studies on the purple hemocyanin indicate that this transformation between the normal blue state and the purple state of oxyhemocyanin is essentially a reversible one. The purple hemocyanin exhibits esr signals that correspond to about one-fifth of the total copper obtained. The results suggest that ethyleneglycol causes a moderate and reversible deformation of the active site of the oxyhemocyanin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|