Connexins regulate calcium signaling by controlling ATP release
| Autor: | M. Nedergaard, Maria Luisa Cotrina, Christian C. Naus, H. Azmi-Ghadimi, Jian Kang, Jiang Li, A. Alves-Rodrigues, Jane H.-C. Lin, Shujun Liu |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Connexin
chemistry.chemical_element Biology Calcium Transfection Connexins Tumor Cells Cultured Animals Humans Channel blocker Cells Cultured Calcium signaling Adenosine Triphosphatases Cerebral Cortex Multidisciplinary Purinergic receptor Gap junction Glioma Biological Sciences Rats Cell biology Microscopy Fluorescence chemistry Astrocytes Connexin 43 Thapsigargin Signal transduction Glioblastoma Intracellular HeLa Cells Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 95:15735-15740 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.95.26.15735 |
| Popis: | Forced expression of gap junction proteins, connexins, enables gap junction-deficient cell lines to propagate intercellular calcium waves. Here, we show that ATP secretion from the poorly coupled cell lines, C6 glioma, HeLa, and U373 glioblastoma, is potentiated 5- to 15-fold by connexin expression. ATP release required purinergic receptor-activated intracellular Ca 2+ mobilization and was inhibited by Cl − channel blockers. Calcium wave propagation also was reduced by purinergic receptor antagonists and by Cl − channel blockers but insensitive to gap junction inhibitors. These observations suggest that cell-to-cell signaling associated with connexin expression results from enhanced ATP release and not, as previously believed, from an increase in intercellular coupling. |
| Databáze: | OpenAIRE |
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