Interaction, uptake, and processing of LbL-coated microcarriers by PMNs
| Autor: | Uta Reibetanz, Sophie Rathmann, Maria Schönberg, Jacqueline Lessig |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Histology
Protamine sulfate Neutrophils Phagocytosis Cell Lipid Bilayers Anti-Inflammatory Agents Pathology and Forensic Medicine Flow cytometry Calcium Carbonate Coated Materials Biocompatible medicine Humans Protamines Lipid bilayer Inflammation Drug Carriers Microscopy Confocal medicine.diagnostic_test Chemistry Dextran Sulfate Microcarrier Cell Biology Flow Cytometry Silicon Dioxide medicine.anatomical_structure Drug delivery Immunology Biophysics Drug carrier medicine.drug |
| Zdroj: | Cytometry. Part A : the journal of the International Society for Analytical Cytology. 79(12) |
| ISSN: | 1552-4930 |
| Popis: | Functionalized microcarriers or hollow capsules transporting active agents offer the opportunity for drug delivery inside cells. A promising application of these drug delivery systems is the direct transport as well as the release of immobilized antiinflammatory substances (AIS) into polymorphonuclear leukocytes (PMNs), which play a key role in the course of inflammatory processes. The intended delivery of AIS into the inflamed tissue could alleviate tissue destruction taking place during chronic inflammation, as well as facilitate the termination of these processes. In this study, the capability of functionalized CaCO(3) microcarriers as AIS transporter system targeted at PMNs is investigated. The time-dependent interaction of protamine sulfate and dextran sulfate multilayer-coated 5 μm ± 1 μm CaCO(3) carriers with PMNs, in comparison with the usage of SiO(2) carriers as monodisperse model system of defined sizes (1, 3, and 5 μm), reveals a sufficient carrier/cell interaction and uptake for coincubation periods between 2 and 24 h. Furthermore, the microcarriers are exposed to an environment simulating primary granule/phagosomal conditions after phagocytosis by means of PMN stimulation. The incubation of CaCO(3) microcarriers with cell supernatant demonstrates a partial multilayer decomposition (three to five layers) within 24 h, allowing the gradual release of AIS within the short PMN life span. This observation suggests a potential application for this drug delivery system inside immunologically active cells and may open the way to new alternatives in the treatment of chronic processes. |
| Databáze: | OpenAIRE |
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