Point-of-care identification of organophosphates in gastric juice by ambient mass spectrometry in emergency settings
| Autor: | You-Da Tsai, Chi-Wei Lee, Yen-Ping Lin, Deng-Chyang Wu, Hung Su, Jentaie Shiea, Ruei-Hao Lee |
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| Rok vydání: | 2018 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Analyte Point-of-Care Systems medicine.medical_treatment Electrospray ionization Clinical Biochemistry Taiwan 010402 general chemistry Mass spectrometry Sensitivity and Specificity 01 natural sciences Biochemistry Specimen Handling chemistry.chemical_compound medicine Humans Detection limit Gastric Juice Chromatography Molecular Structure 010401 analytical chemistry Biochemistry (medical) Organophosphate Parts-per notation General Medicine Gastric lavage Healthy Volunteers Organophosphates 0104 chemical sciences chemistry Chlorpyrifos Emergency Service Hospital |
| Zdroj: | Clinica Chimica Acta. 485:288-297 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2018.07.002 |
| Popis: | Background For emergency management, it is important to promptly identify the organophosphate ingested by self-poisoning patients since different organophosphates cause intoxication through different mechanisms and require different therapeutic strategies. This study aimed at the development of a point-of-care ambient mass spectrometric approach for rapid identification of organophosphate(s) in gastric juice for emergency management. Methods Six organophosphate insecticides that are commonly ingested by self-poisoning patients in Taiwan were examined. The sample solutions were prepared and diluted with human gastric juice. A direct metallic probe was dipped and removed immediately from the sample solution. The probe was then inserted into the thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS) to detect the analyte on the probe. Results Since no pretreatment of the specimen was required, the sampling processes followed by thermal desorption-electrospray ionization and mass spectrometric analysis of the organophosphate in the gastric juice were completed within 30 s. The detection limit of the organophosphates is at the 10–100 parts per billion level. Good linearity was observed between the corresponding changes in mass spectrometric signal intensities and the changes of organophosphate concentrations within the range of 5–1000 parts per billion. The high efficiency of this ambient mass spectrometric platform was further confirmed when a real sample of the drained gastric lavage fluid of a patient who suffered from ingestion of chlorpyrifos was collected in the emergency room and tested with this cutting-edge technique. Conclusions The results suggested that TD-ESI/MS is promising in promptly providing toxicological information to assist succeeding medical management in an emergency room. |
| Databáze: | OpenAIRE |
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