LC/MS at the whole protein level: Studies of biomolecular structure and interactions using native LC/MS and cross-path reactive chromatography (XP-RC) MS
| Autor: | Honglin Yao, Wenhua Yang, Igor A. Kaltashov, Andrei Lipatnikov, Khaja Muneeruddin, Jake W. Pawlowski, Cedric E. Bobst |
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| Rok vydání: | 2018 |
| Předmět: |
Bioanalysis
Chromatography Chemistry Protein Conformation Electrospray ionization 010401 analytical chemistry Size-exclusion chromatography Ion chromatography Proteins Biomolecular structure 010402 general chemistry Chromatography Ion Exchange Ligands 01 natural sciences General Biochemistry Genetics and Molecular Biology Mass Spectrometry 0104 chemical sciences Gas phase Liquid chromatography–mass spectrometry Protein Interaction Domains and Motifs Protein Interaction Maps Molecular Biology Higher Order Structure |
| Zdroj: | Methods (San Diego, Calif.). 144 |
| ISSN: | 1095-9130 |
| Popis: | Interfacing liquid chromatography (LC) with electrospray ionization (ESI) to enable on-line MS detection had been initially implemented using reversed phase LC, which in the past three decades remained the default type of chromatography used for LC/MS and LC/MS/MS studies of protein structure. In contrast, the advantages of other types of LC as front-ends for ESI MS, particularly those that allow biopolymer higher order structure to be preserved throughout the separation process, enjoyed relatively little appreciation until recently. However, the past few years witnessed a dramatic surge of interest in the so-called "native" (with "non-denaturing" being perhaps a more appropriate adjective) LC/MS and LC/MS/MS analyses within the bioanalytical and biophysical communities. This review focuses on recent advances in this field, with an emphasis on size exclusion and ion exchange chromatography as front-end platforms for protein characterization by LC/MS. Also discussed are the benefits provided by the integration of chemical reactions in the native LC/MS analyses, including both ion chemistry in the gas phase (e.g., limited charge reduction for characterization of highly heterogeneous biopolymers) and solution-phase reactions (using the recently introduced technique cross-path reactive chromatography). |
| Databáze: | OpenAIRE |
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