Laminarin improves developmental competence of porcine early stage embryos by inhibiting oxidative stress
| Autor: | Xuerui Yao, Bao Yuan, Xing-Hui Shen, Yong-Xun Jin, Jia-Bao Zhang, Shuang Liang, Nam-Hyung Kim, Hao Jiang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sus scrofa Embryonic Development Gene Expression Biology medicine.disease_cause Andrology Embryo Culture Techniques 03 medical and health sciences Laminarin chemistry.chemical_compound Food Animals SOX2 medicine Animals Blastocyst Small Animals Glucans YAP1 Membrane Potential Mitochondrial Equine Embryogenesis Embryo Hydrogen Peroxide Glutathione Oxidative Stress 030104 developmental biology medicine.anatomical_structure chemistry embryonic structures Maternal to zygotic transition Animal Science and Zoology Female Reactive Oxygen Species Oxidative stress |
| Zdroj: | Theriogenology. 115 |
| ISSN: | 1879-3231 |
| Popis: | Laminarin (LMA), a β-glucan mixture with good biocompatibility, improves the growth performance and immune response when used as food additives and nutraceuticals. The aim of the present research was to explore the effects of LMA on porcine early stage embryo development, as well as the underlying mechanisms. The results showed that the developmental competence of porcine early stage embryos was dramatically improved after LMA supplementation during the in vitro culture period. The presence of 20 μg/mL LMA during the in vitro culture period significantly improved cleavage rate, blastocyst formation rates, hatching rate, and total cell number in the blastocyst compared to that in the control group. Notably, LMA attenuated the intracellular reactive oxygen species generation induced by H2O2. Furthermore, LMA not only increased intracellular glutathione levels, but also ameliorated mitochondrial membrane potential. In addition, the expression of a zygotic genome activation related gene (YAP1), pluripotency-related genes (OCT4, NANOG, and SOX2), and hatching-related genes (COX2, GATA4, and ITGA5) were up-regulated following LMA supplementation during porcine early stage embryo development. These results demonstrate that LMA has beneficial effects on the development of porcine early stage embryos via regulation of oxidative stress. This evidence provides a novel method for embryo development improvement associated with exposure to LMA. |
| Databáze: | OpenAIRE |
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