Alterations in synaptic proteins and their encoding mRNAs in prefrontal cortex in schizophrenia: a possible neurochemical basis for 'hypofrontality'
| Autor: | K O Schluterman, W. Q. Sturner, Robert E. Mrak, J. G. Sheng, W. S. T. Griffin, Craig N. Karson |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Psychosis Synaptosomal-Associated Protein 25 Transcription Genetic Central nervous system Synaptophysin Prefrontal Cortex Hypofrontality Nerve Tissue Proteins Biology Synaptic vesicle Cellular and Molecular Neuroscience Neurochemical Reference Values Glial Fibrillary Acidic Protein medicine Humans RNA Messenger Prefrontal cortex Molecular Biology Aged Aged 80 and over Glial fibrillary acidic protein Membrane Proteins Middle Aged medicine.disease Psychiatry and Mental health medicine.anatomical_structure Synapses biology.protein Schizophrenia Female Autopsy Neuroscience |
| Zdroj: | Molecular psychiatry. 4(1) |
| ISSN: | 1359-4184 |
| Popis: | An impairment of prefrontal cortical functioning in schizophrenia ('hypofrontality') has been suggested by clinical, neuroimaging, and postmortem brain tissue studies. We used Western immunoblot and Northern hybridization analyses of postmortem brain tissue obtained from 14 schizophrenic patients and 12 control patients of similar ages to measure tissue levels of synaptophysin (a structural synaptic vesicle protein) and of SNAP-25 (a 25-kDa presynaptic protein), and their encoding mRNAs, in Brodmann's area 10 of prefrontal cortex. There were significant decreases in tissue levels of both of these proteins in prefrontal cortex of schizophrenic patients relative to controls. In contrast, tissue levels for the mRNAs encoding these proteins were not decreased in schizophrenic patients. Subsequent labeling of the same Western immunoblots showed no difference in tissue levels of glial fibrillary acidic protein (GFAP) in schizophrenic and control patients. Similarly, subsequent hybridization of the same Northern hybridization membranes showed no difference in tissue levels of GFAP mRNA or of 28S rRNA in schizophrenic and control patients. These alterations in tissue levels of synaptophysin and SNAP-25 are consistent with the idea that the clinically observed 'hypofrontality' of schizophrenia arises from abnormalities of synaptic number or structural integrity in prefrontal cortex. |
| Databáze: | OpenAIRE |
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