A Japanese trial to monitor methicillin-resistant Staphylococcus aureus (MRSA) in imported swine during the quarantine period

Autor: Yusuke Nakahara, Hisae Fukuhara, Mariko Uchiyama, Minako Furuno, Kei Uenoyama, Shigeru Morino, Mayumi Kijima
Rok vydání: 2018
Předmět:
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
Microbiology (medical)
Veterinary medicine
Swine
030106 microbiology
Immunology
Erythromycin
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Polymerase Chain Reaction
Microbiology
Methicillin
03 medical and health sciences
Antibiotic resistance
Japan
Communicable Diseases
Imported
Drug Resistance
Multiple
Bacterial
Ampicillin
Prevalence
medicine
Animals
Immunology and Allergy
One Health
Swine Diseases
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Ciprofloxacin
030104 developmental biology
Staphylococcus aureus
Quarantine
Multilocus sequence typing
Gentamicin
Multilocus Sequence Typing
medicine.drug
Zdroj: Journal of Global Antimicrobial Resistance. 14:182-184
ISSN: 2213-7165
DOI: 10.1016/j.jgar.2018.03.012
Popis: Objectives This study aimed to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in imported swine during the quarantine period in Japan. Methods Nasal swabs from a total of 125 swine belonging to 15 lots (unit of import) from five countries were investigated for MRSA from July 2016 to February 2017. Two isolates per positive lot were chosen for multilocus sequence typing (MLST). PCR was performed to determine the presence of the czrC gene, and antimicrobial susceptibility testing was performed by the broth dilution method. Results MRSA isolates were obtained from six lots (41 heads; 32.8%) from two countries. All 12 isolates that underwent MLST (two per positive lot) were classified as ST398, harboured the czrC gene and were resistant to ampicillin and tetracycline; some isolates showed additional resistance to erythromycin or streptomycin, but resistance to ciprofloxacin, gentamicin or chloramphenicol was not observed. Conclusions MRSA ST398 isolates were obtained from imported swine in this first trial to monitor MRSA during the quarantine period in Japan. For the ‘One Health’ approach against antimicrobial resistance, monitoring imported animals and generating feedback data would be important.
Databáze: OpenAIRE
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