Correlation between various trace elements and ultramicroscopic structure of epiretinal macular membranes and glial cells

Autor: Romano MR, Cennamo G, Montorio D, Del Prete S, Ferrara M, Cennamo G., CENNAMO, GILDA
Přispěvatelé: Romano, Mr, Cennamo, G, Montorio, D, Del Prete, S, Ferrara, M, Cennamo, G., Cennamo, Gilda
Rok vydání: 2018
Předmět:
Male
Pathology
genetic structures
medicine.medical_treatment
lcsh:Medicine
Vitrectomy
Mineral composition
Spectrum analysis techniques
Diagnostic Radiology
chemistry.chemical_compound
0302 clinical medicine
Medicine and Health Sciences
Electron Microscopy
Prospective Studies
lcsh:Science
Tomography
Microscopy
Multidisciplinary
Chemistry
Radiology and Imaging
Phosphorus
Epiretinal Membrane
Middle Aged
Zinc
Membrane
medicine.anatomical_structure
Physical Sciences
Female
Scanning Electron Microscopy
Tomography
Optical Coherence
Research Article
Chemical Elements
Pars plana
medicine.medical_specialty
Imaging Techniques
Iron
Research and Analysis Methods
03 medical and health sciences
stomatognathic system
Diagnostic Medicine
medicine
Humans
Energy dispersive X-ray spectroscopy
Aged
Glial activation
Diabetic Retinopathy
Biochemistry
Genetics and Molecular Biology (all)
Spectrum Analysis
Sodium
lcsh:R
Electron beam spectrum analysis techniques
Retinal
biochemical phenomena
metabolism
and nutrition
Trace Elements
Agricultural and Biological Sciences (all)
Microscopy
Electron
Scanning
030221 ophthalmology & optometry
Ultrastructure
lcsh:Q
Sulfur
030217 neurology & neurosurgery
Zdroj: PLoS ONE, Vol 13, Iss 9, p e0204497 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: INTRODUCTION: Elements such as zinc, iron, copper, sulphur and phosphorus have been identified in retinal layers and implicated in vital retinal functions. Regarding mineral composition of epiretinal membranes (ERMs), literature is lacking. This study aimed to analyze both mineral composition and anatomical ultrastructure of ERMs to clarify the pathophysiology of this disease. METHODS: Twenty ERMs (10 diabetic ERMs and 10 idiopathic ERMs) from 20 patients were harvested during pars plana vitrectomy. Scanning Electron Microscopy (SEM) was used to investigate the anatomical ultrastructure of the peeled ERMs. Mineral composition was analyzed using energy-dispersive spectrometry (EDS). The most frequent elements were evaluated in relation to appearance of ERMs analyzed at SEM and at OCT images. RESULTS: Sulphur was the most frequent element found (in 80% of the samples), followed by sodium (50%) and phosphorus (45%). The presence of these elements was not significantly different between diabetic and idiopathic ERMs (P >0.05). Using SEM we found a folded tissue in all ERMs, except in 4 ERMs, where we observed only a smooth tissue. There was a trend of sodium to be more frequent in ERMs with folded layers at SEM examination. CONCLUSIONS: Several elements were identified in ERMs, and sulphur, sodium and phosphorus were the most frequent ones. This finding may help to understand their role in the physiopatology of epiretinal proliferation and in glial activation.
Databáze: OpenAIRE
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