Correlation between various trace elements and ultramicroscopic structure of epiretinal macular membranes and glial cells
Autor: | Romano MR, Cennamo G, Montorio D, Del Prete S, Ferrara M, Cennamo G., CENNAMO, GILDA |
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Přispěvatelé: | Romano, Mr, Cennamo, G, Montorio, D, Del Prete, S, Ferrara, M, Cennamo, G., Cennamo, Gilda |
Rok vydání: | 2018 |
Předmět: |
Male
Pathology genetic structures medicine.medical_treatment lcsh:Medicine Vitrectomy Mineral composition Spectrum analysis techniques Diagnostic Radiology chemistry.chemical_compound 0302 clinical medicine Medicine and Health Sciences Electron Microscopy Prospective Studies lcsh:Science Tomography Microscopy Multidisciplinary Chemistry Radiology and Imaging Phosphorus Epiretinal Membrane Middle Aged Zinc Membrane medicine.anatomical_structure Physical Sciences Female Scanning Electron Microscopy Tomography Optical Coherence Research Article Chemical Elements Pars plana medicine.medical_specialty Imaging Techniques Iron Research and Analysis Methods 03 medical and health sciences stomatognathic system Diagnostic Medicine medicine Humans Energy dispersive X-ray spectroscopy Aged Glial activation Diabetic Retinopathy Biochemistry Genetics and Molecular Biology (all) Spectrum Analysis Sodium lcsh:R Electron beam spectrum analysis techniques Retinal biochemical phenomena metabolism and nutrition Trace Elements Agricultural and Biological Sciences (all) Microscopy Electron Scanning 030221 ophthalmology & optometry Ultrastructure lcsh:Q Sulfur 030217 neurology & neurosurgery |
Zdroj: | PLoS ONE, Vol 13, Iss 9, p e0204497 (2018) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | INTRODUCTION: Elements such as zinc, iron, copper, sulphur and phosphorus have been identified in retinal layers and implicated in vital retinal functions. Regarding mineral composition of epiretinal membranes (ERMs), literature is lacking. This study aimed to analyze both mineral composition and anatomical ultrastructure of ERMs to clarify the pathophysiology of this disease. METHODS: Twenty ERMs (10 diabetic ERMs and 10 idiopathic ERMs) from 20 patients were harvested during pars plana vitrectomy. Scanning Electron Microscopy (SEM) was used to investigate the anatomical ultrastructure of the peeled ERMs. Mineral composition was analyzed using energy-dispersive spectrometry (EDS). The most frequent elements were evaluated in relation to appearance of ERMs analyzed at SEM and at OCT images. RESULTS: Sulphur was the most frequent element found (in 80% of the samples), followed by sodium (50%) and phosphorus (45%). The presence of these elements was not significantly different between diabetic and idiopathic ERMs (P >0.05). Using SEM we found a folded tissue in all ERMs, except in 4 ERMs, where we observed only a smooth tissue. There was a trend of sodium to be more frequent in ERMs with folded layers at SEM examination. CONCLUSIONS: Several elements were identified in ERMs, and sulphur, sodium and phosphorus were the most frequent ones. This finding may help to understand their role in the physiopatology of epiretinal proliferation and in glial activation. |
Databáze: | OpenAIRE |
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