Expression of Na/K-ATPase subunits in the human cochlea: a confocal and super-resolution microscopy study with special reference to auditory nerve excitation and cochlear implantation
| Autor: | Charlotta Kämpfe Nordström, Anneliese Schrott-Fischer, Maria Luque, Helge Rask-Andersen, Wei Liu, Niklas Danckwardt-Lillieström, Rudolf Glueckert |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Gene isoform
Sodium Confocal cochlea lcsh:Medicine chemistry.chemical_element structured illumination microscopy 030209 endocrinology & metabolism In Vitro Techniques law.invention Mice 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Auditory nerve Confocal microscopy law otorhinolaryngologic diseases Animals Humans Medicine Na na/k-atpase human Na+/K+-ATPase Cochlear Nerve Cochlea Ion transporter Microscopy Confocal 030219 obstetrics & reproductive medicine Super-resolution microscopy business.industry lcsh:R Neurosciences Articles General Medicine Cochlear Implantation Immunohistochemistry Acoustic Stimulation K-ATPase chemistry Biophysics sense organs Sodium-Potassium-Exchanging ATPase business auditory nerve Neurovetenskaper |
| Zdroj: | Upsala Journal of Medical Sciences, Vol 124, Iss 3, Pp 168-179 (2019) Upsala Journal of Medical Sciences |
| ISSN: | 2000-1967 0300-9734 |
| DOI: | 10.1080/03009734.2019.1653408 |
| Popis: | Background: For the first time the expression of the ion transport protein sodium/potassium-ATPase and its isoforms was analyzed in the human cochlea using light- and confocal microscopy as well as super-resolution structured illumination microscopy. It may increase our understanding of its role in the propagation and processing of action potentials in the human auditory nerve and how electric nerve responses are elicited from auditory prostheses. Material and methods: Archival human cochlear sections were obtained from trans-cochlear surgeries. Antibodies against the Na/K-ATPase beta 1 isoform together with alpha 1 and alpha 3 were used for immunohistochemistry. An algorithm was applied to assess the expression in various domains. Results: Na/K ATPase beta 1 subunit was expressed, mostly combined with the alpha 1 isoform. Neurons expressed the beta 1 subunit combined with alpha 3, while satellite glial cells expressed the alpha 1 isoform without recognized association with beta 1. Types I and II spiral ganglion neurons and efferent fibers expressed the Na/K-ATPase alpha 3 subunit. Inner hair cells, nerve fibers underneath, and efferent and afferent fibers in the organ of Corti also expressed alpha 1. The highest activity of Na/K-ATPase beta 1 was at the inner hair cell/nerve junction and spiral prominence. Conclusion: The human auditory nerve displays distinct morphologic features represented in its molecular expression. It was found that electric signals generated via hair cells may not go uninterrupted across the spiral ganglion, but are locally processed. This may be related to particular filtering properties in the human acoustic pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |