Absorption and efficiency of insulin after oral administration of insulin-loaded nanocapsules in diabetic rats
| Autor: | Fabienne Cournarie, D Chevenne, M. Seiller, Christine Vauthier, D. Auchère, Bernard Lacour |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Pharmaceutical Science Biological Availability Nanocapsules Dosage form Diabetes Mellitus Experimental Pharmacokinetics Oral administration Internal medicine Diabetes mellitus Hyperinsulinism Blood plasma medicine Animals Hypoglycemic Agents Insulin Pancreatic hormone Drug Implants business.industry medicine.disease Microspheres Rats Endocrinology Intestinal Absorption business |
| Zdroj: | International journal of pharmaceutics. 242(1-2) |
| ISSN: | 0378-5173 |
| Popis: | Poly(isobutylcyanoacrylate) nanocapsules have been shown to decrease the blood glucose level after oral administration to streptozotocin-induced diabetic fasted rats after 2 days [Diabetes 37 (1988) 246]. Yet, the absorption of insulin in the blood of rats has not been characterised. The aim of this work was to evaluate the biological activity of insulin given orally as nanocapsules. Humalog®-loaded nanocapsules (50 IU/kg) were administered by gavage to streptozotocin-induced diabetic rats. Thirty minutes to 1 h after oral administration, significant levels of human insulin were detected in rat plasma. However, the concentrations were very heterogenous from one rat to another and no decrease of glycemia could be observed. In addition, parenteral injection of insulin in solution showed that high levels of the protein are necessary to decrease blood glucose concentration in diabetic rats. These concentrations were not reached after oral administration. The same dose of insulin decreased glycemia by 50% in normal rats and by only 25% in diabetics. This suggested that an insulino-resistance was developed by streptozotocin-induced diabetic rats. |
| Databáze: | OpenAIRE |
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