Bovine leukaemia virus-induced lymphocytosis in sheep is associated with reduction of spontaneous B cell apoptosis
| Autor: | Schwartz-Cornil, Isabelle, Chevallier, N, Belloc, C, Le Rhun, D, Laine, V., Berthelemy, M, Mateo, A, Levy, D., Lainé, V |
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| Přispěvatelé: | Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire - Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Mount Sinai Hospital [Toronto, Canada] (MSH), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Transcription
Genetic Lymphocytosis Cell Survival [SDV]Life Sciences [q-bio] viruses Lymphocyte Apoptosis Polymerase Chain Reaction Peripheral blood mononuclear cell Virus 03 medical and health sciences 0302 clinical medicine Reference Values immune system diseases Virology Leukemia Virus Bovine medicine Animals Humans RNA Messenger Cells Cultured B cell ComputingMilieux_MISCELLANEOUS 030304 developmental biology [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology B-Lymphocytes Human T-lymphotropic virus 1 0303 health sciences Sheep biology Cell Cycle Enzootic Bovine Leukosis Cell cycle Flow Cytometry biology.organism_classification medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.IMM]Life Sciences [q-bio]/Immunology Cattle medicine.symptom 030215 immunology |
| Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 1997, pp.153-162 Journal of General Virology, Microbiology Society, 1997, 78 (1), pp.153-162. ⟨10.1099/0022-1317-78-1-153⟩ |
| ISSN: | 0022-1317 1465-2099 |
| DOI: | 10.1099/0022-1317-78-1-153⟩ |
| Popis: | Experimental inoculation of sheep with bovine leukaemia virus (BLV), a retrovirus homologous to the human T-lymphotropic virus type 1 (HTLV-1), induces a chronic expansion of the B lymphocyte population (persistent lymphocytosis) and the development of a B cell leukaemia/lymphosarcoma syndrome. To gain insight into the mechanisms of BLV-induced lymphocytosis, we tested B cell survival capacity and cycling activity in peripheral blood mononuclear cells (PBMCs) from lymphocytotic, asymptomatic and control sheep. Interestingly, B cells from lymphocytotic sheep presented a lower level of spontaneous apoptosis (29%) in ex vivo cultures compared to that obtained with infected asymptomatic (42%) and control (57%/o) sheep PBMCs. Virus capsid (CA) synthesis was mainly found among surviving B cells and the percentage of CA-producing B cells correlated with the extent of B cell survival, indicating that BLV replication in B lymphocytes may promote protection from cell death. B cell survival was not linked with increases in expression of Bcl-2 mRNA or membrane leukosialin (CD43), although both are documented to be involved in some aspects of the B cell life-span. Finally, cell cycle analyses in freshly isolated PBMCs from lymphocytotic sheep revealed a slightly increased proportion of B cells in S phase compared to controls. Altogether, these data suggest that both BLV-induced B cell proliferation and extended survival are involved in the lymphocytotic stage encountered in BLV infection in sheep. |
| Databáze: | OpenAIRE |
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