Insights into the DNA sequence elements required for partitioning and copy number control of the yeast 2-micron plasmid
| Autor: | Melanie J. Dobson, Mary E. McQuaid, Anastasiia Mereshchuk |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins DNA Copy Number Variations Locus (genetics) Saccharomyces cerevisiae Biology DNA sequencing Chromosome segregation 03 medical and health sciences Plasmid Chromosome Segregation Genetics DNA Fungal 030304 developmental biology Repetitive Sequences Nucleic Acid 0303 health sciences Base Sequence Plasmid partitioning 030302 biochemistry & molecular biology Promoter General Medicine Yeast Trans-Activators Selfish DNA Cell Division Plasmids |
| Zdroj: | Current genetics. 65(4) |
| ISSN: | 1432-0983 |
| Popis: | The yeast 2-micron plasmid is an almost perfect selfish DNA. The entire coding capacity of the plasmid is dedicated to ensuring its own inheritance, with no benefit to its host. Despite high copy number, the plasmid confers no phenotype. It manages this feat by possessing mechanisms for plasmid copy-number control and for partitioning. The former increases plasmid numbers when they fall, but is repressed at high copy number, while the latter ensures 2-micron copies are equally partitioned during host cell division. Although the plasmid amplification mechanism is well established, the partitioning system and the means by which the 2-micron plasmid partitioning proteins, Rep1 and Rep2, regulate plasmid copy number remain incompletely understood. This review focuses on recent efforts to determine the nature of Rep protein complexes formed at the plasmid stability locus (STB) and at plasmid gene promoters, the identity of DNA sequence elements required for Rep protein association, and the mechanism by which the Rep proteins manage their dual roles of plasmid partitioning and plasmid gene repression. |
| Databáze: | OpenAIRE |
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