Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows
| Autor: | Alessandra Bridi, Natália Marins Bastos, Luciano Andrade Silva, Cibele M. Prado, Guilherme Pugliesi, Flávio Vieira Meirelles, Felipe Perecin, Gabriella Mamede Andrade, Jorge Enrique Díaz Pinzón, Maite del Collado, Juliano Coelho da Silveira, Rosane Mazzarella |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell signaling animal structures medicine.medical_treatment Biology small extracellular vesicles Andrology reproduction 03 medical and health sciences 0302 clinical medicine BOVINOS microRNA medicine oviductal cells Gene Original Research 030219 obstetrics & reproductive medicine lcsh:Veterinary medicine General Veterinary bovine Embryogenesis Embryo Embryonic stem cell oviductal fluid embryo maternal-communication 030104 developmental biology miRNAs Oviduct lcsh:SF600-1100 Ovulation induction Veterinary Science |
| Zdroj: | Frontiers in Veterinary Science, Vol 8 (2021) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Frontiers in Veterinary Science |
| ISSN: | 2297-1769 |
| DOI: | 10.3389/fvets.2021.639752/full |
| Popis: | Early embryonic development occurs in the oviduct, where an ideal microenvironment is provided by the epithelial cells and by the oviductal fluid produced by these cells. The oviductal fluid contains small extracellular vesicles (sEVs), which through their contents, including microRNAs (miRNAs), can ensure proper cell communication between the mother and the embryo. However, little is known about the modulation of miRNAs within oviductal epithelial cells (OECs) and sEVs from the oviductal fluid in pregnant cows. In this study, we evaluate the miRNAs profile in sEVs from the oviductal flushing (OF-sEVs) and OECs from pregnant cows compared to non-pregnant, at 120 h after ovulation induction. In OF-sEVs, eight miRNAs (bta-miR-126-5p, bta-miR-129, bta-miR-140, bta-miR-188, bta-miR-219, bta-miR-345-3p, bta-miR-4523, and bta-miR-760-3p) were up-regulated in pregnant and one miRNA (bta-miR-331-5p) was up-regulated in non-pregnant cows. In OECs, six miRNAs (bta-miR-133b, bta-miR-205, bta-miR-584, bta-miR-551a, bta-miR-1193, and bta-miR-1225-3p) were up-regulated in non-pregnant and none was up-regulated in pregnant cows. Our results suggest that embryonic maternal communication mediated by sEVs initiates in the oviduct, and the passage of gametes and the embryo presence modulate miRNAs contents of sEVs and OECs. Furthermore, we demonstrated the transcriptional levels modulation of selected genes in OECs in pregnant cows. Therefore, the embryonic-maternal crosstalk potentially begins during early embryonic development in the oviduct through the modulation of miRNAs in OECs and sEVs in pregnant cows. |
| Databáze: | OpenAIRE |
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