Strain differences in muscarinic cholinergic receptor antagonism of fat intake and acquisition and expression of fat-conditioned flavor preferences in male BALB/c, C57BL/6 and SWR mice
Autor: | Julia Iskhakova, Gabriela Fazilov, Nicole Locurto, Petra Dohnalova, Merna Shenouda, Richard J. Bodnar, Asnat Yuabov, Nicole Franz, Ben Iskhakov, Tatjana Mustac, Jason Macanian, Emanuel Israel |
---|---|
Rok vydání: | 2019 |
Předmět: |
Male
C57BL/6 medicine.medical_specialty medicine.drug_class Conditioning Classical Scopolamine Clinical Biochemistry Administration Oral Muscarinic Antagonists Toxicology Biochemistry BALB/c Food Preferences Mice Behavioral Neuroscience chemistry.chemical_compound Internal medicine Muscarinic acetylcholine receptor medicine Animals Receptor Saccharin Phospholipids Biological Psychiatry Pharmacology Mice Inbred BALB C biology Receptor antagonist biology.organism_classification Receptors Muscarinic Soybean Oil Flavoring Agents Mice Inbred C57BL Endocrinology chemistry Taste NMDA receptor Emulsions Antagonism |
Zdroj: | Pharmacology Biochemistry and Behavior. 187:172792 |
ISSN: | 0091-3057 |
Popis: | Murine strain differences occur for both intakes of and preferences for sugars and fats. Previous studies demonstrated that the muscarinic cholinergic receptor antagonist, scopolamine (SCOP) more potently reduced sucrose and saccharin intakes in inbred C57BL/6 and BALB/c than SWR mice, sucrose-conditioned flavor preferences (CFP) expression in BALB/c, but not C57BL/6 or SWR mice, and sucrose-CFP acquisition in BALB/c relative to SWR and C57BL/6 mice. Although fat intake and fat-CFP are observed in all three strains, strain-specific effects were previously observed following dopamine D1, opiate and NMDA receptor antagonism of sweet and fat intake and CFP. The present study investigated whether muscarinic receptor antagonism differentially affected fat (Intralipid) intake and preferences in these strains by examining whether SCOP altered fat (Intralipid) intake and fat-CFP expression and acquisition in BALB/c, C57BL/6 and SWR mice. SCOP (0.1–10 mg/kg) significantly reduced Intralipid (5%) intake in all three strains across 2 h. In fat-CFP expression experiments, food-restricted mice consumed one flavored (conditioned stimulus (CS)+, 5 sessions) Intralipid (5%) solution and a differently-flavored (CS−, 5 sessions) Intralipid (0.5%) solution. Two-bottle CS choice tests with the two flavors mixed in 0.5% Intralipid occurred following vehicle and two SCOP doses (1, 5 mg/kg). SCOP elicited small, but significant reductions in fat-CFP expression in BALB/c and C57BL/6, but not SWR mice. In fat-CFP acquisition experiments, separate groups of BALB/c, C57BL/6 and SWR mice were treated prior to the ten acquisition training sessions with vehicle or two SCOP (2.5, 5 mg/kg) doses followed by six two-bottle choice tests without injections. SCOP eliminated fat-CFP acquisition in all three strains. Thus, muscarinic receptor signaling mediates learning, and to a lesser degree maintenance of fat-CFP while maximally inhibiting fat intake in the three strains. |
Databáze: | OpenAIRE |
Externí odkaz: |