Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering
| Autor: | Mahetab H. Amer, Sumana Shrestha, Francesco Pappalardo, Cristina González-García, Felicity R. A. J. Rose, Salah Abdelrazig, Jane McLaren, David Needham, Cameron Alexander, Sara Trujillo, Kevin M. Shakesheff, Morgan R. Alexander, Manuel Salmerón-Sánchez, Jing Qian Wong, Lee A. Stevens, Marta Alvarez-Paino, Jong Bong Lee, Dong-Hyun Kim |
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| Rok vydání: | 2020 |
| Předmět: |
Bone development
Cellular differentiation Biophysics Bioengineering 02 engineering and technology Biomaterials 03 medical and health sciences In vivo Osteogenesis Gene expression Humans Bone regeneration Cells Cultured 030304 developmental biology 0303 health sciences Tissue Engineering Chemistry Regeneration (biology) Stem Cells Mesenchymal stem cell Microcarrier Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Cell biology Stem cell fate Mechanics of Materials Ceramics and Composites 0210 nano-technology |
| Zdroj: | Biomaterials. 266 |
| ISSN: | 1878-5905 0142-9612 |
| Popis: | Mesenchymal stem cells have been the focus of intense research in bone development and regeneration. We demonstrate the potential of microparticles as modulating moieties of osteogenic response by utilizing their architectural features. Topographically textured microparticles of varying microscale features were produced by exploiting phase-separation of a readily-soluble sacrificial component from polylactic acid. The influence of varying topographical features on primary human mesenchymal stem cell attachment, proliferation and markers of osteogenesis was investigated. In the absence of osteoinductive supplements, cells cultured on textured microparticles exhibited notably increased expression of osteogenic markers relative to conventional smooth microparticles. They also exhibited varying morphological, attachment and proliferation responses. Significantly altered gene expression and metabolic profiles were observed, with varying histological characteristicsin vivo. This study highlights how tailoring topographical design offers cell-instructive 3D microenvironments which allow manipulation of stem cell fate by eliciting the desired downstream response without use of exogenous osteoinductive factors. |
| Databáze: | OpenAIRE |
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