Ovine ill-thrift in Nova Scotia. 5. The production and toxicology of chetomin, a metabolite of Chaetomium spp
| Autor: | W. A. Jerram, R M Archibald, Leo C. Vining, A. Taylor, C K Leach, D. Brewer, C M Christensen, Stephen Safe, R G Stevenson, J. M. Duncan, C J Mirocha |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Turkeys
medicine.drug_class Metabolite Immunology Antibiotics Administration Oral Sheep Diseases Biology Zea mays Applied Microbiology and Biotechnology Microbiology Median lethal dose Corn steep liquor Lethal Dose 50 Toxicology chemistry.chemical_compound Ascomycota Species Specificity Genetics medicine Animals Humans Molecular Biology Soil Microbiology Mycelium Sheep Bacteria Body Weight Fungi Drug Resistance Microbial General Medicine Spores Fungal Chaetomium biology.organism_classification Anti-Bacterial Agents Culture Media Neoplasm Proteins Rats Spore Nova Scotia chemistry Antibacterial activity HeLa Cells |
| Zdroj: | Canadian Journal of Microbiology. 18:1129-1137 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m72-174 |
| Popis: | Chetomin is a metabolite of Chaetomium cochliodes and C. globosum. It is produced by C. cochliodes on a denned medium and the yield is increased about 50-fold by the addition of corn steep liquor to the culture medium. Five single spore isolates of C. cochliodes (HLX 440) all produced chetomin and one of them produced appreciable quantities of a metabolite related to chetomin but having a S:N ratio of 1:1. Previous reports of the antibacterial activity of chetomin were confirmed and the antibiotic was shown, in addition, to inhibit the mycelial growth of some fungi. At 0.02 μg/ml, it inhibited protein synthesis in cultures of HeLa cells. Its oral LD50 in rats was 75 mg/kg, and in turkeys, 30 mg/kg. No pathological changes were observed in lambs dosed orally at 30 mg/kg but a single intraperitoneal dose of chetomin at 5 mg/kg resulted in the death of half the animals treated. Sublethal oral doses in all the animal species mentioned resulted in loss of body weight. |
| Databáze: | OpenAIRE |
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