Single-molecule imaging of a three-component ordered actin disassembly mechanism
| Autor: | Silvia Jansen, Jeff Gelles, Agnieszka Collins, Bruce L. Goode, Casey A. Ydenberg, Samantha M. Chin |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cofilin 1
General Physics and Astronomy Arp2/3 complex macromolecular substances Filamentous actin Article General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Escherichia coli Animals Humans Actin-binding protein 030304 developmental biology 0303 health sciences Multidisciplinary biology Microfilament Proteins Actin remodeling General Chemistry Cofilin Actin cytoskeleton 3. Good health Cell biology Actin Cytoskeleton Microscopy Fluorescence ras GTPase-Activating Proteins Formins biology.protein Rabbits MDia1 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | The mechanisms by which cells destabilize and rapidly disassemble filamentous actin networks have remained elusive; however, Coronin, Cofilin and AIP1 have been implicated in this process. Here using multi-wavelength single-molecule fluorescence imaging, we show that mammalian Cor1B, Cof1 and AIP1 work in concert through a temporally ordered pathway to induce highly efficient severing and disassembly of actin filaments. Cor1B binds to filaments first, and dramatically accelerates the subsequent binding of Cof1, leading to heavily decorated, stabilized filaments. Cof1 in turn recruits AIP1, which rapidly triggers severing and remains bound to the newly generated barbed ends. New growth at barbed ends generated by severing was blocked specifically in the presence of all three proteins. This activity enabled us to reconstitute and directly visualize single actin filaments being rapidly polymerized by formins at their barbed ends while simultanteously being stochastically severed and capped along their lengths, and disassembled from their pointed ends. The roles of Coronin, Cofilin and AIP1 in promoting actin disassembly have not been well understood. Here using single-molecule fluorescence imaging, Jansen et al. show that the three proteins act together in a coordinated, temporal pathway to induce rapid severing and disassembly of actin filaments. |
| Databáze: | OpenAIRE |
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