Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen
| Autor: | Henning Jørgensen, Lisbeth Olsson, Birgitte Rønnow, Eva Maria Akke Palmqvist |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Nitrogen
Saccharomyces cerevisiae Carbon starvation chemistry.chemical_element Biology Applied Microbiology and Biotechnology Fungal Proteins chemistry.chemical_compound medicine Biomass Starvation Glycogen Trehalose General Medicine biology.organism_classification Yeast Carbon chemistry Biochemistry Fermentation medicine.symptom Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 59(2-3) |
| ISSN: | 0175-7598 |
| Popis: | An industrial strain of Saccharomyces cerevisiae (DGI 342) was cultivated in fed-batch cultivations at a specific growth rate of 0.2 h(-1). The yeast was then exposed to carbon or nitrogen starvation for up to 8 h, to study the effect of starvation on fermentative capacity and content of protein, trehalose and glycogen. Nitrogen starvation triggered the accumulation of trehalose and glycogen. After 8 h of starvation, the content of trehalose and glycogen was increased 4-fold and 2-fold, respectively. Carbon starvation resulted in a partial conversion of glycogen into trehalose. The trehalose content increased from 45 to 64 mg (g dry-weight)(-1), whereas the glycogen content in the same period was reduced from 55 to 5 mg (g dry-weight)(-1). Glycogen was consumed faster than trehalose during storage of the starved yeast for 1 month. Nitrogen starvation resulted in a decrease in the protein content of the yeast cells, and the fermentative capacity per gram dry-weight decreased by 40%. The protein content in the carbon-starved yeast increased as a result of starvation due to the fact that the content of glycogen was reduced. The fermentative capacity per gram dry-weight was, however, unaltered. |
| Databáze: | OpenAIRE |
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