Differential expression of GABAA receptor π subunit in cultured rat alveolar epithelial cells
| Autor: | Lin Liu, Telugu Narasaraju, Nili Jin, Narasaiah Kolliputi, Jiwang Chen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Histology Protein subunit medicine.medical_treatment Cell Culture Techniques Biology Pathology and Forensic Medicine Rats Sprague-Dawley chemistry.chemical_compound GABA receptor Internal medicine medicine Animals RNA Messenger Receptor Lung Cells Cultured gamma-Aminobutyric Acid Messenger RNA Matrigel GABAA receptor Respiration Growth factor Cell Membrane Cell Differentiation Epithelial Cells Cell Biology Receptors GABA-A Rats Up-Regulation Cell biology Pulmonary Alveoli Protein Subunits Endocrinology Gene Expression Regulation chemistry Keratinocyte growth factor |
| Zdroj: | Cell and Tissue Research. 321:173-183 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-005-1130-8 |
| Popis: | Although type A gamma-aminobutyric acid (GABA) receptors (ligand-gated Cl(-) channels) have been extensively studied in the central nervous system, no information is available on this receptor in lung cells. We have examined the expression of GABA(A) receptor pi-subunit (GABRP) during the trans-differentiation between rat alveolar epithelial type II cells and type I cells. Rat alveolar type II cells, when cultured on plastic plates, gradually trans-differentiated into type-I-like cells and lost their GABRP mRNA expression. However, the GABRP mRNA was partially retained in the type II cells cultured on Matrigel. Keratinocyte growth factor (a mitogen of type II cells) increased GABRP expression. A detached collagen gel maintained the GABRP mRNA to a level close to that of the freshly isolated type II cells. An air-liquid interface culture system, mimicking in vivo conditions in the lung, significantly up-regulated the expression of GABRP mRNA and protein. mRNAs of the GABA(A) receptor alpha1-, alpha3-, beta2-, gamma2-, and gamma3-subunits were also detected in rat type II cells. These results suggest that GABRP expression is differentially regulated by culture substrata, growth factor, detached gel, and an air-apical surface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink