Stimulation of polydnavirus replication by 20-hydroxyecdysone
| Autor: | B. A. Webb, M. D. Summers |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
DNA Replication
Virus Integration Molecular Sequence Data Restriction Mapping 20-Hydroxyecdysone Insect Viruses Biology Virus Replication Polymerase Chain Reaction DNA sequencing Virus Cellular and Molecular Neuroscience chemistry.chemical_compound Animals Molecular Biology Gene Pharmacology Ecdysteroid Base Sequence DNA Superhelical Polydnavirus fungi Pupa DNA replication Cell Biology biology.organism_classification Hymenoptera Virology Cell biology Lepidoptera Ecdysterone Oligodeoxyribonucleotides chemistry Viral replication DNA Viral Molecular Medicine Female |
| Zdroj: | Experientia. 48:1018-1022 |
| ISSN: | 1420-9071 0014-4754 |
| Popis: | During oviposition the endoparasitic wasp Campoletis sonorensis, introduces a polydnavirus into parasitized insects where viral gene expression is required for endoparasite survival. The polydnavirus is integrated into wasp chromosomal DNA and replicates only in the ovary. Ecdysteroids regulate the developmental expression of many insect genes and may regulate polydnavirus replication. Direct verification of viral replication was performed by dot blot hybridization and by amplifying DNA sequences containing the viral integration site; this 'junction' fragment cannot be amplified from integrated virus. Thoracic ligations and in vitro ecdysteroid treatments of wasp ovaries support the hypothesis that polydnavirus DNA replication is regulated by ecdysteroid during parasite development. |
| Databáze: | OpenAIRE |
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