Mapping of host range mutants of bacteriophage φX174
| Autor: | G.A. van Arkel, Peter Weisbeek, J.H. van de Pol |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Mutant
DNA Single-Stranded Spheroplasts medicine.disease_cause Coliphages Bacteriophage chemistry.chemical_compound Virology Centrifugation Density Gradient Escherichia coli medicine Allele Gene Genetics Mutation biology DNA Viruses Temperature Chromosome Mapping Nucleic Acid Hybridization biology.organism_classification Molecular biology Genes chemistry DNA Viral Shigella DNA Circular DNA |
| Zdroj: | Virology. 52:408-416 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(73)90335-8 |
| Popis: | Three independently isolated host range mutants of phage φX174 are localized in gene G. This lends further support to the idea that the gene G product is the main protein involved in the adsorption process. The mapping was performed by hybridizing fragments of the RF DNA of the host range mutants with circular single-stranded DNA of suppressor-sensitive or temperature-sensitive mutants from seven cistrons. Different fragment lengths were used and the amount of cohybridization of the h mutation with the sus+ or ts+ allele was a measure for the distance between the two markers. It is shown that the same method can be applied to localize every marker under investigation. |
| Databáze: | OpenAIRE |
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