Folic acid utilisation related to sulfa drug resistance in Saccharomyces cerevisiae
| Autor: | Carol Hopkins Sibley, Ian Macreadie, Eleanor G. Hankins, Onisha Patel, Ann M Bayly, Laura A. Castelli, Peter Coloe, Janette Berglez |
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| Rok vydání: | 2001 |
| Předmět: |
Sulfamethoxazole
Saccharomyces cerevisiae Microbial Sensitivity Tests Microbiology Folinic acid chemistry.chemical_compound Folic Acid Anti-Infective Agents Drug Resistance Fungal Genetics medicine Molecular Biology Dihydrofolate synthase chemistry.chemical_classification biology biology.organism_classification Enzyme chemistry Biochemistry Mutation Antifolate biology.protein Dihydrofolic acid Dihydropteroate synthase medicine.drug |
| Zdroj: | FEMS Microbiology Letters. 204:387-390 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | Saccharomyces cerevisiae mutants deficient in folate synthesis have been constructed and employed to study the utilisation of exogenous folates in yeast. One mutant specifically lacked dihydropteroate synthase while the second lacked dihydrofolate synthase. Exogenous folinic acid restored optimal growth to both strains. Folic acid did not generally rescue growth but spontaneous isolates capable of utilising folic acid were selected. The folic acid synthesis pathway in the folate utilising isolates was restored via transformation with FOL1 or FOL3 expression plasmids and transformants were tested for resistance to sulfamethoxazole (SMX). The presence of elevated levels of folic acid led to greatly reduced SMX sensitivity regardless of whether strains were folate utilisers or not. |
| Databáze: | OpenAIRE |
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