Experimental study of vascular endothelial growth factor gene therapy for avascular necrosis of the femoral head
Autor: | Wei-hua Xu, Yang Cao, Xiong Yufang, Du Jingyuan, Yang Shu-hua, Jin Li |
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Rok vydání: | 2003 |
Předmět: |
Vascular Endothelial Growth Factor A
Pathology medicine.medical_specialty Angiogenesis Genetic enhancement Biomedical Engineering Neovascularization Physiologic Avascular necrosis Bone tissue Biochemistry Biomaterials Femoral head chemistry.chemical_compound Random Allocation Implants Experimental Femur Head Necrosis Osteogenesis Genetics medicine Animals Earth-Surface Processes business.industry Gene Transfer Techniques Femur Head Transfection Genetic Therapy medicine.disease Recombinant Proteins Vascular endothelial growth factor medicine.anatomical_structure chemistry Immunohistochemistry Collagen Rabbits business Plasmids |
Zdroj: | Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 23(3) |
ISSN: | 1672-0733 |
Popis: | To explore a new method for the therapy of the avascular necrosis of the femoral head, the recombinant plasmid pCD-hVEGF165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly. |
Databáze: | OpenAIRE |
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