Characterization of the Brazilian endemic clone of methicillin-resistant Staphylococcus aureus (MRSA) from hospitals throughout Brazil
| Autor: | Juarez Braz de Faria, Carlos Emílio Levy, Elsa Masae Mamizuka, Geraldo A. Oliveira |
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| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Staphylococcus aureus Tetracycline lcsh:QR1-502 Erythromycin MRSA Microbial Sensitivity Tests Biology medicine.disease_cause lcsh:Microbiology Microbiology lcsh:Infectious and parasitic diseases Hospitals Private Brazilian endemic clone parasitic diseases medicine Pulsed-field gel electrophoresis Humans lcsh:RC109-216 Bacteriophage Typing Phage typing Staphylococus aureus Cross Infection Infection Control Hospitals Public biochemical phenomena metabolism and nutrition Staphylococcal Infections bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Lincomycin Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Ciprofloxacin Infectious Diseases epidemiology Methicillin Resistance Brazil medicine.drug |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 5, Iss 4, Pp 163-170 (2001) Brazilian Journal of Infectious Diseases, Vol 5, Iss 4, Pp 163-170 Brazilian Journal of Infectious Diseases, Volume: 5, Issue: 4, Pages: 163-170, Published: AUG 2001 Brazilian Journal of Infectious Diseases v.5 n.4 2001 Brazilian Journal of Infectious Diseases Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
| ISSN: | 1413-8670 |
| Popis: | The objective of this study was to characterize patterns of the Brazilian endemic clone of methicillin-resistant Staphylococcus aureus (MRSA) from hospitals throughout Brazil. We studied 83 MRSA strains isolated from patients hospitalized in 27 public and private hospitals in 19 cities located in 14 Brazilian states from September, 1995, to June, 1997. The MRSA strains were typed using antibiograms, bacteriophage typing and pulsed field gel electrophoresis (PFGE). The analysis of genomic DNA by PFGE showed that 65 isolates presented the same PFGE pattern. This pattern was present in all of the hospitals studied indicating the presence of an endemic MRSA clone widely disseminated throughout Brazilian hospitals (BEC). All isolates belonging to the BEC proved to be resistant to ciprofloxacin, erythromycin, lincomycin, trimethoprim-sulphamethoxazole, and tetracycline. Variable susceptibility to these drugs was found only in isolates belonging to clones other than the BEC. The results show that, among MRSA, the BEC is common in Brazil. The best method for mapping changes in the frequency of this clone among MRSA is pulsed field gel electrophoresis. Use of molecular mapping is an important tool for monitoring the spread of potentially dangerous microbes. |
| Databáze: | OpenAIRE |
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