Global identification of the genetic networks andcis-regulatory elements of the cold response in zebrafish
| Autor: | Wanying Zhai, Zhichao Wu, Peng Hu, Bingshe Han, Yimeng Liu, Dong Zhang, Mingli Liu, Jinfeng Wang, Yu Shen, Liangbiao Chen, Hongbo Niu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Gene regulatory network Biology Cell Line Protein Interaction Mapping Genetics Animals Gene Regulatory Networks Nucleotide Motifs Binding site Promoter Regions Genetic Zebrafish Transcription factor Gene Regulation of gene expression Cold-Shock Response Gene Expression Profiling Gene regulation Chromatin and Epigenetics biology.organism_classification Cold shock response Gene expression profiling Gene Ontology Gene Expression Regulation Organ Specificity Female Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The transcriptional programs of ectothermic teleosts are directly influenced by water temperature. However, the cis- and trans-factors governing cold responses are not well characterized. We profiled transcriptional changes in eight zebrafish tissues exposed to mildly and severely cold temperatures using RNA-Seq. A total of 1943 differentially expressed genes (DEGs) were identified, from which 34 clusters representing distinct tissue and temperature response expression patterns were derived using the k-means fuzzy clustering algorithm. The promoter regions of the clustered DEGs that demonstrated strong co-regulation were analysed for enriched cis-regulatory elements with a motif discovery program, DREME. Seventeen motifs, ten known and seven novel, were identified, which covered 23% of the DEGs. Two motifs predicted to be the binding sites for the transcription factors Bcl6 and Jun, respectively, were chosen for experimental verification, and they demonstrated the expected cold-induced and cold-repressed patterns of gene regulation. Protein interaction modeling of the network components followed by experimental validation suggested that Jun physically interacts with Bcl6 and might be a hub factor that orchestrates the cold response in zebrafish. Thus, the methodology used and the regulatory networks uncovered in this study provide a foundation for exploring the mechanisms of cold adaptation in teleosts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|