Kidney protection by hypothermic total liquid ventilation after cardiac arrest in rabbits

Autor: Mourad Chenoune, Alain Berdeaux, Sébastien Giraud, Fanny Lidouren, Matthias Kohlhauer, Jean-Michel Goujon, Lys Darbera, Renaud Tissier, Patrick Bruneval, Bijan Ghaleh, Thierry Hauet, Nathalie Quellard, Béatrice Fernandez, Sandrine Pons
Přispěvatelé: INSERM U955, équipe 3, Pharmacie-Toxicologie, École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques ( IRTOMIT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Poitiers, Service de Biochimie [Poitiers], Centre hospitalier universitaire de Poitiers (CHU Poitiers), Laboratoire de Recherche en Imagerie : Méthodes d'imagerie des Échanges transcapillaires (LRI - EA4062), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), This study was supported by a grant (ABYSS- R12031JJ) from the 'Agence Nationale pour la Recherche' (Paris, France). It was also suported by Inserm, Université de Poitiers, Région Poitou-Charentes, Conseil général de la Vienne, Région Ile-de-France (CODDIM), University Paris Est Créteil and CHU de Poitiers. R. Tissier was a recipient of a 'Contrat d'Interface Inserm- ENV'. M. Kohlhauer was supported by a doctoral fellowship from the 'Region Ile-de France'., École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut Mondor de Recherche Biomédicale (IMRB), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Rok vydání: 2013
Předmět:
Resuscitation
MESH: Heart Arrest
Myocardial ischemia
Liquid Ventilation
Renal function
MESH: Rabbits
030204 cardiovascular system & hematology
Kidney
Kidney Function Tests
Article
03 medical and health sciences
0302 clinical medicine
Hypothermia
Induced
medicine
Animals
MESH: Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Treatment Outcome
MESH: Kidney Diseases
MESH: Hypothermia
Induced
MESH: Liquid Ventilation
business.industry
030208 emergency & critical care medicine
MESH: Kidney
Hypothermia
3. Good health
Heart Arrest
Disease Models
Animal
Anesthesiology and Pain Medicine
medicine.anatomical_structure
Treatment Outcome
MESH: Kidney Function Tests
Anesthesia
Total Liquid Ventilation
Hypot
Kidney Diseases
Rabbits
medicine.symptom
MESH: Disease Models
Animal
business
Cardiovascular outcomes
Zdroj: Anesthesiology
Anesthesiology, Lippincott, Williams & Wilkins, 2014, 120 (4), pp.861-9. ⟨10.1097/ALN.0000000000000048⟩
ISSN: 1528-1175
0003-3022
Popis: Background: Total liquid ventilation (TLV) with perfluorocarbons has been shown to induce rapid protective cooling in animal models of myocardial ischemia and cardiac arrest, with improved neurological and cardiovascular outcomes after resuscitation. In this study, the authors hypothesized that hypothermic TLV can also limit kidney injury after cardiac arrest. Methods: Anesthetized rabbits were submitted to 15 min of untreated ventricular fibrillation. After resuscitation, three groups of eight rabbits each were studied such as (1) life support plus hypothermia (32°–33°C) induced by cold TLV (TLV group), (2) life support without hypothermia (control group), and (3) Sham group (no cardiac arrest). Life support was continued for 6 h before euthanasia and kidney removal. Results: Time to target esophageal temperature was less than 5 min in the TLV group. Hypothermia was accompanied by preserved renal function in the TLV group as compared with control group regarding numerous markers including creatinine blood levels (12 ± 1 vs. 16 ± 2 mg/l, respectively; mean ± SEM), urinary N-acetyl-β-(d)-glucosaminidase (1.70 ± 0.11 vs. 3.07 ± 0.10 U/mol of creatinine), γ-glutamyltransferase (8.36 ± 0.29 vs. 12.96 ± 0.44 U/mol of creatinine), or β2-microglobulin (0.44 ± 0.01 vs. 1.12 ± 0.04 U/mol of creatinine). Kidney lesions evaluated by electron microscopy and conventional histology were also attenuated in TLV versus control groups. The renal-protective effect of TLV was not related to differences in delayed inflammatory or immune renal responses because transcriptions of, for example, interferon-γ, tumor necrosis factor-α, interleukin-1β, monocyte chemoattractant protein-1, toll-like receptor-2, toll-like receptor-4, and vascular endothelial growth factor were similarly altered in TLV and control versus Sham. Conclusion: Ultrafast cooling with TLV is renal protective after cardiac arrest and resuscitation, which could increase kidney availability for organ donation.
Databáze: OpenAIRE
Externí odkaz: