Intronic Polymorphisms in the CDKN2B-AS1 Gene Are Strongly Associated with the Risk of Myocardial Infarction and Coronary Artery Disease in the Saudi Population

Autor: J. Francis Borgio, Mohammed Fakhry Ibrahim, Mohammed Almansori, Ekaterina V Baranova, Rudaynah A. Alali, Abdullah M. Alshehri, Bobby P. C. Koeleman, Abdullah Al-Ali, Mohammed S. Al-Madan, Fahad Al-Muhanna, Awatif N. Al-Nafie, Sayed AbdulAzeez, Amein K. Al-Ali, Folkert W. Asselbergs, Brendan J. Keating
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Genome-wide association study
030204 cardiovascular system & hematology
CDKN2B-AS1 gene
lcsh:Chemistry
single nucleotide polymorphisms
0302 clinical medicine
Non-U.S. Gov't
lcsh:QH301-705.5
Spectroscopy
Genetics
education.field_of_study
Research Support
Non-U.S. Gov't
General Medicine
Middle Aged
3. Good health
Computer Science Applications
coronary artery disease
myocardial infarction
Saudi Arabia
Female
RNA
Long Noncoding
Adult
medicine.medical_specialty
Population
Single-nucleotide polymorphism
Research Support
Polymorphism
Single Nucleotide
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Journal Article
Humans
Physical and Theoretical Chemistry
education
Molecular Biology
Genotyping
Genetic association
Aged
business.industry
Organic Chemistry
Haplotype
Case-control study
Odds ratio
Introns
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Case-Control Studies
business
Zdroj: International Journal of Molecular Sciences, Vol 17, Iss 3, p 395 (2016)
International Journal of Molecular Sciences; Volume 17; Issue 3; Pages: 395
International Journal of Molecular Sciences
International journal of molecular sciences, 17(3). Multidisciplinary Digital Publishing Institute (MDPI)
ISSN: 1422-0067
Popis: Recent genome-wide association studies identified single nucleotide polymorphisms (SNPs) on the chromosome 9p21.3 conferring the risk for CAD (coronary artery disease) in individuals of Caucasian ancestry. We performed a genetic association study to investigate the effect of 12 candidate SNPs within 9p21.3 locus on the risk of CAD in the Saudi population of the Eastern Province of Saudi Arabia. A total of 250 Saudi CAD patients who had experienced an myocardial infarction (MI) and 252 Saudi age-matched healthy controls were genotyped using TaqMan assay. Controls with evidenced lack of CAD provided 90% of statistical power at the type I error rate of 0.05. Five percent of the results were rechecked for quality control using Sanger sequencing, the results of which concurred with the TaqMan genotyping results. Association analysis of 12 SNPs indicated a significant difference in the genotype distribution for four SNPs between cases and controls (rs564398 p = 0.0315, χ² = 4.6, odds ratio (OD) = 1.5; rs4977574 p = 0.0336, χ² = 4.5, OD = 1.4; rs2891168 p = 1.85 × 10 - 10, χ² = 40.6, OD = 2.1 and rs1333042 p = 5.14 × 10 - 9, χ² = 34.1, OD = 2.2). The study identified three protective haplotypes (TAAG p = 1.00 × 10 - 4; AGTA p = 0.022 and GGGCC p = 0.0175) and a risk haplotype (TGGA p = 2.86 × 10 - 10) for the development of CAD. This study is in line with others that indicated that the SNPs located in the intronic region of the CDKN2B-AS1 gene are associated with CAD.
Databáze: OpenAIRE
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