Intronic Polymorphisms in the CDKN2B-AS1 Gene Are Strongly Associated with the Risk of Myocardial Infarction and Coronary Artery Disease in the Saudi Population
Autor: | J. Francis Borgio, Mohammed Fakhry Ibrahim, Mohammed Almansori, Ekaterina V Baranova, Rudaynah A. Alali, Abdullah M. Alshehri, Bobby P. C. Koeleman, Abdullah Al-Ali, Mohammed S. Al-Madan, Fahad Al-Muhanna, Awatif N. Al-Nafie, Sayed AbdulAzeez, Amein K. Al-Ali, Folkert W. Asselbergs, Brendan J. Keating |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Male Genome-wide association study 030204 cardiovascular system & hematology CDKN2B-AS1 gene lcsh:Chemistry single nucleotide polymorphisms 0302 clinical medicine Non-U.S. Gov't lcsh:QH301-705.5 Spectroscopy Genetics education.field_of_study Research Support Non-U.S. Gov't General Medicine Middle Aged 3. Good health Computer Science Applications coronary artery disease myocardial infarction Saudi Arabia Female RNA Long Noncoding Adult medicine.medical_specialty Population Single-nucleotide polymorphism Research Support Polymorphism Single Nucleotide Catalysis Article Inorganic Chemistry 03 medical and health sciences Internal medicine medicine Journal Article Humans Physical and Theoretical Chemistry education Molecular Biology Genotyping Genetic association Aged business.industry Organic Chemistry Haplotype Case-control study Odds ratio Introns 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Case-Control Studies business |
Zdroj: | International Journal of Molecular Sciences, Vol 17, Iss 3, p 395 (2016) International Journal of Molecular Sciences; Volume 17; Issue 3; Pages: 395 International Journal of Molecular Sciences International journal of molecular sciences, 17(3). Multidisciplinary Digital Publishing Institute (MDPI) |
ISSN: | 1422-0067 |
Popis: | Recent genome-wide association studies identified single nucleotide polymorphisms (SNPs) on the chromosome 9p21.3 conferring the risk for CAD (coronary artery disease) in individuals of Caucasian ancestry. We performed a genetic association study to investigate the effect of 12 candidate SNPs within 9p21.3 locus on the risk of CAD in the Saudi population of the Eastern Province of Saudi Arabia. A total of 250 Saudi CAD patients who had experienced an myocardial infarction (MI) and 252 Saudi age-matched healthy controls were genotyped using TaqMan assay. Controls with evidenced lack of CAD provided 90% of statistical power at the type I error rate of 0.05. Five percent of the results were rechecked for quality control using Sanger sequencing, the results of which concurred with the TaqMan genotyping results. Association analysis of 12 SNPs indicated a significant difference in the genotype distribution for four SNPs between cases and controls (rs564398 p = 0.0315, χ² = 4.6, odds ratio (OD) = 1.5; rs4977574 p = 0.0336, χ² = 4.5, OD = 1.4; rs2891168 p = 1.85 × 10 - 10, χ² = 40.6, OD = 2.1 and rs1333042 p = 5.14 × 10 - 9, χ² = 34.1, OD = 2.2). The study identified three protective haplotypes (TAAG p = 1.00 × 10 - 4; AGTA p = 0.022 and GGGCC p = 0.0175) and a risk haplotype (TGGA p = 2.86 × 10 - 10) for the development of CAD. This study is in line with others that indicated that the SNPs located in the intronic region of the CDKN2B-AS1 gene are associated with CAD. |
Databáze: | OpenAIRE |
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