Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil

Autor: Sandra Curiel-Alegre, Blanca Velasco-Arroyo, Carlos Rumbo, Aqib Hassan Ali Khan, Juan Antonio Tamayo-Ramos, Carlos Rad, José Luis R. Gallego, Rocío Barros
Rok vydání: 2022
Předmět:
History
Environmental Engineering
Polymers and Plastics
Health
Toxicology and Mutagenesis
Soil enzymes
Industrial and Manufacturing Engineering
Total petroleum hydrocarbons polluted soils
Soil
Bioaugmentation
Alkanes
Environmental Chemistry
Soil Pollutants
Micronutrients
Polycyclic Aromatic Hydrocarbons
Business and International Management
Materials
Phospholipids
Soil Microbiology
Minerals
Materiales
Biostimulation
Bioaugmentation
Phospholipid fatty acids
Total petroleum hydrocarbons polluted soils
Soil enzymes
Fatty Acids
Public Health
Environmental and Occupational Health
Phosphorus
General Medicine
General Chemistry
Phenanthrenes
Pollution
Hydrocarbons
Phosphoric Monoester Hydrolases
Biostimulation
Biodegradation
Environmental
Petroleum
Phospholipid fatty acids
Potassium
Oils
Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes
Peptide Hydrolases
Zdroj: Scopus
ISSN: 1556-5068
Popis: In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation’ efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic Hydrocarbons (PAHs) were less abundant in this soil, results also decreased, especially for the most abundant, the phenanthrene. Despite improving the degradation rates, results revealed that recalcitrant and hydrophobic petroleum compounds remained unchanged, indicating that mobility, linked to bioavailability, probably represents the limiting step for further soil recovery.
This work is funded by the GREENER project of the European Union’s Horizon 2020 research and innovation program (Grant Agreement No. 826312). S. Curiel pre-doctoral contract was funded by Junta de Castilla y Leon ´ (ORDEN EDU/1508/2020, de 15 de diciembre).
Databáze: OpenAIRE
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