EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice
| Autor: | Hindol Gupta, Manidipa Banerjee, K. Bandopadhyay, J. Mitra, Puja Singh, Geethu Emily Thomas, Steny Simon, Raja Paul, Sabyasachi Sutradhar, Tapas Manna, Binshad Badarudeen, M. R. Renjith, K. K. Gireesh |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromosome movement Microtubule-associated protein Chromosomal Proteins Non-Histone Science General Physics and Astronomy Mitosis macromolecular substances Biology Microscopy Atomic Force Microtubules General Biochemistry Genetics and Molecular Biology Article Chromosome segregation 03 medical and health sciences Microtubule Chromosome Segregation Chlorocebus aethiops Animals Humans Amino Acid Sequence Kinetochores Multidisciplinary COS cells Microscopy Confocal Sequence Homology Amino Acid Kinetochore fungi General Chemistry Cell biology 030104 developmental biology COS Cells RNA Interference Astral microtubules Microtubule-Associated Proteins HeLa Cells |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Kinetochore couples chromosome movement to dynamic microtubules, a process that is fundamental to mitosis in all eukaryotes but poorly understood. In vertebrates, spindle-kinetochore-associated (Ska1–3) protein complex plays an important role in this process. However, the proteins that stabilize Ska-mediated kinetochore-microtubule attachment remain unknown. Here we show that microtubule plus-end tracking protein EB1 facilitates Ska localization on microtubules in vertebrate cells. EB1 depletion results in a significant reduction of Ska1 recruitment onto microtubules and defects in mitotic chromosome alignment, which is also reflected in computational modelling. Biochemical experiments reveal that EB1 interacts with Ska1, facilitates Ska1-microtubule attachment and together stabilizes microtubules. Structural studies reveal that EB1 either with Ska1 or Ska complex forms extended structures on microtubule lattice. Results indicate that EB1 promotes Ska association with K-fibres and facilitates kinetochore-microtubule attachment. They also implicate that in vertebrates, chromosome coupling to dynamic microtubules could be mediated through EB1-Ska extended structures. Ska1 is a kinetochore-localised protein that couples kinetochore movement to microtubule (MT) depolymerisation. Here Thomas et al. show that the MT +TIP binding protein EB1 recruits Ska1 to the MT-kinetochore interface and stabilises the interaction between Ska1 and MTs. |
| Databáze: | OpenAIRE |
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