Osmotic Regulation Is Required for Cancer Cell Survival under Solid Stress
| Autor: | Michelle R. Dawson, Nithin Ravikumar, Daniel J. McGrail, Quang Minh N. Kieu, Kathleen M. McAndrews, Chandler P. Brandenburg |
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| Rok vydání: | 2015 |
| Předmět: |
Azides
Osmosis Sodium-Hydrogen Exchangers Cell Survival Sodium Cell Culture Techniques Biophysics chemistry.chemical_element Breast Neoplasms macromolecular substances Adenocarcinoma Models Biological Cell Line Tumor Humans Cytoskeleton Cation Transport Proteins Actin Sodium-Hydrogen Exchanger 1 Tissue Scaffolds Biophysical Letter Sodium channel Spheroid Actins Biomechanical Phenomena Cell biology chemistry Cancer cell Tonicity Female Guanosine Triphosphate Intracellular |
| Zdroj: | Biophysical Journal. 109:1334-1337 |
| ISSN: | 0006-3495 |
| Popis: | For a solid tumor to grow, it must be able to support the compressive stress that is generated as it presses against the surrounding tissue. Although the literature suggests a role for the cytoskeleton in counteracting these stresses, there has been no systematic evaluation of which filaments are responsible or to what degree. Here, using a three-dimensional spheroid model, we show that cytoskeletal filaments do not actively support compressive loads in breast, ovarian, and prostate cancer. However, modulation of tonicity can induce alterations in spheroid size. We find that under compression, tumor cells actively efflux sodium to decrease their intracellular tonicity, and that this is reversible by blockade of sodium channel NHE1. Moreover, although polymerized actin does not actively support the compressive load, it is required for sodium efflux. Compression-induced cell death is increased by both sodium blockade and actin depolymerization, whereas increased actin polymerization offers protective effects and increases sodium efflux. Taken together, these results demonstrate that cancer cells modulate their tonicity to survive under compressive solid stress. |
| Databáze: | OpenAIRE |
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