Recovery of Indigenous probiotic Lactobacillus plantarum Mut-7 on healthy Indonesian adults after consumption of fermented milk containing these bacteria
Autor: | Endang Sutriswati Rahayu, E Zulaichah, F H Pamungkaningtyas, S Nurfiani, I N Sujaya, Achmad Dinoto, Jaka Widada, Tyas Utami, Pratama Nur Hasan, Muhammad Nur Cahyanto, Mariyatun Mariyatun, Mohammad Juffrie, I A Harahap |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0303 health sciences
biology 030306 microbiology food and beverages Gut flora biology.organism_classification 16S ribosomal RNA law.invention 03 medical and health sciences Probiotic fluids and secretions Starter law bacteria Ingestion Original Article Food science Lactobacillus plantarum Bacteria Feces 030304 developmental biology Food Science |
Zdroj: | J Food Sci Technol |
Popis: | Probiotics are live microorganisms that confer health benefits on the host when administered in adequate amounts, such as to support the balance of gut microbiota. In this study, the selected indigenous probiotic strain, Lactobacillus plantarum Mut-7, was used as a starter culture to produce fermented milk. A total of 28 healthy Indonesian youngsters and adults (male/female: 16/12; age 19.4–22.9 years old; normal BMI range 18.6–22.7 kg/m(2)) were supplemented with the fermented milk. This study aimed to determine the recovery of L. plantarum Mut-7 through molecular analysis from the subjects feces after ingestion of 140 mL fermented milk containing 7.0 log(10) CFU/ml of L. plantarum Mut-7 for 15 days. Molecular detection was performed using the rep-PCR technique and sequencing of DNA 16S rRNA. Consumption of fermented milk containing L. plantarum Mut-7 enabled reduction of total E. coli and Coliform non-E. coli in several subjects. It was able to increase the total LAB and total L. plantarum in subjects’ feces. The number of L. plantarum and mesophilic LAB increased by 5.5 ± 1.6 log(10) CFU/g, 1.8 ± 0.8 log(10). On the other side, thermophilic LAB increased by 2.8 ± 3.0 log(10) CFU/g in 23 out of 28 subjects. These findings proved that L. plantarum survived in the human gastrointestinal tract. Based on the molecular identification technique using rep-PCR technique and sequencing of gene 16S rRNA, two isolates had similarity to L. plantarum Mut-7 by a coefficient value of 100%. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13197-021-05046-z. |
Databáze: | OpenAIRE |
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