Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy
| Autor: | Deog-Su Park, Jang-Su Park, Yoon-Bo Shim, Farzana Darain |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Carboxylic acid
Biomedical Engineering Biophysics Biosensing Techniques Antibodies Vitellogenins Vitellogenin Electrochemistry Carp Immunoassay chemistry.chemical_classification Detection limit Chromatography biology Spectrum Analysis Temperature General Medicine Quartz crystal microbalance Hydrogen-Ion Concentration biology.organism_classification Dielectric spectroscopy Linear range chemistry biology.protein Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 19:1245-1252 |
| ISSN: | 0956-5663 |
| Popis: | A simple and direct immunosensor for the determination of carp ( Carassius auratus ) vitellogenin (Vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. The monoclonal antibody specific to carp ( C. auratus ) Vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. The antibody immobilization and antibody–antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectroscopic techniques. The impedance change occurred at the sensor surface due to the specific immuno-interaction was utilized to determine Vtg. The sensor showed high selectivity and sensitive response to Vtg in a buffered medium without redox probe. Vtg was determined in the linear range from 1.0 to 8.0 μg/l with the standard deviation of ±0.13 ( n =3) and the detection limit was determined to be 0.42 μg/l. This method was applied to the determination of Vtg in real male and female carp ( C. auratus ) serum samples. |
| Databáze: | OpenAIRE |
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