Pectin coatings on titanium alloy scaffolds produced by additive manufacturing:Promotion of human bone marrow stromal cell proliferation
| Autor: | Cosmin Mihai Cotrut, Roman A. Surmenev, Jagoda Żydek, Julia A. H. Kaeswurm, M. Buchweitz, Ute Hempel, Alina Vladescu, Maria A. Surmeneva, Elżbieta Pamuła, Timothy E.L. Douglas, Krzysztof Pietryga, Andrey Koptyug |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Stromal cell
food.ingredient Materials science Pectin Mechanical Engineering 0206 medical engineering Biomaterial 02 engineering and technology Adhesion 021001 nanoscience & nanotechnology Condensed Matter Physics Bone tissue Cell morphology 020601 biomedical engineering food medicine.anatomical_structure Mechanics of Materials medicine Biophysics Alkaline phosphatase General Materials Science 0210 nano-technology Cell adhesion |
| DOI: | 10.1016/j.matlet.2018.05.060 |
| Popis: | Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation. |
| Databáze: | OpenAIRE |
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