Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults
Autor: | Bruno Lemaitre, Ryu Ueda, Sheila Vidal, Kaoru Saigo, François Leulier |
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Rok vydání: | 2002 |
Předmět: |
Male
Fas-Associated Death Domain Protein Biology General Biochemistry Genetics and Molecular Biology Animals Genetically Modified Transactivation Gene expression Gram-Negative Bacteria Animals Drosophila Proteins Gene Death domain Adaptor Proteins Signal Transducing RNA Double-Stranded MAP kinase kinase kinase Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) fungi Signal Transducing Signal transducing adaptor protein RNA Adaptor Proteins Antibacterial Response Molecular biology Gene Expression Regulation Insect Proteins Drosophila Female General Agricultural and Biological Sciences Carrier Proteins |
Zdroj: | Current biology : CB. 12(12) |
ISSN: | 0960-9822 |
Popis: | In Drosophila, the immune deficiency (Imd) pathway controls antibacterial peptide gene expression in the fat body in response to Gram-negative bacterial infection [1, 2]. The ultimate target of the Imd pathway is Relish, a transactivator related to mammalian P105 and P100 NF-κB precursors [3]. Relish is processed in order to translocate to the nucleus, and this cleavage is dependent on both Dredd, an apical caspase related to caspase-8 of mammals, and the fly Iκ-B kinase complex (dmIKK) [4–9]. dTAK1, a MAPKKK, functions upstream of the dmIKK complex and downstream of Imd, a protein with a death domain similar to that of mammalian receptor interacting protein (RIP) [10, 11]. Finally, the peptidoglycan recognition protein-LC (PGRP-LC) acts upstream of Imd and probably functions as a receptor for the Imd pathway [12–14]. Using inducible expression of dFADD double-stranded RNA, we demonstrate that dFADD is a novel component of the Imd pathway: dFADD double-stranded RNA expression reduces the induction of antibacterial peptide-encoding genes after infection and renders the fly susceptible to Gram-negative bacterial infection. Epistatic studies indicate that dFADD acts between Imd and Dredd. Our results reinforce the parallels between the Imd and the TNF-R1 pathways. |
Databáze: | OpenAIRE |
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