Polymorphisms in human pre-miRNAs
| Autor: | Naoharu Iwai, Hiroaki Naraba |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Genetics
Lin-4 microRNA precursor Polymorphism Genetic Base Sequence Pre mirnas Molecular Sequence Data Biophysics Cell Biology Biology Blotting Northern Non-coding RNA Biochemistry Genome Blot MicroRNAs Sequence Homology Nucleic Acid Gene expression microRNA RNA Precursors Nucleic acid Humans Nucleic Acid Conformation Molecular Biology |
| Zdroj: | Biochemical and Biophysical Research Communications. 331:1439-1444 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2005.04.051 |
| Popis: | MicroRNAs constitute a growing class of non-coding RNAs that are thought to regulate gene expression via translational repression. MicroRNAs are initially transcribed as several hundred-nucleotide pri-miRNAs and are then processed to approximately 60-nucleotide hairpin pre-miRNAs. We hypothesized that polymorphisms in both pre-miRNA and mature microRNA modify various biological processes by influencing the processing and/or target selection of microRNAs. In the present study, we sequenced 173 human pre-miRNA genome regions in 96 subjects and found 10 polymorphisms in the 10 pre-miRNA hairpin regions. Although most of these polymorphisms seem to have no effect on microRNA processing, we identified a C to A polymorphism in the mature miR-30c-2 sequence. This polymorphism may alter target selection and thus exert profound biological effects. To the best of our knowledge, this is the first report of polymorphisms in pre-miRNAs. |
| Databáze: | OpenAIRE |
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