Exploring the metabolic fate of medium-chain triglycerides in healthy individuals using a stable isotope tracer
| Autor: | Lodewijk IJlst, Loek L. Crefcoeur, Riekelt H. Houtkooper, Jeannette C. Bleeker, Frédéric M. Vaz, Sacha Ferdinandusse, Johannes B. van Goudoever, Dewi van Harskamp, Henk Schierbeek, Suzan J. G. Knottnerus, Frits A. Wijburg, Ronald J.A. Wanders, Gepke Visser, Mirjam Langeveld |
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| Přispěvatelé: | Graduate School, Laboratory Genetic Metabolic Diseases, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, APH - Methodology, APH - Quality of Care, ARD - Amsterdam Reproduction and Development, General Paediatrics, Neonatology, ACS - Diabetes & metabolism, Endocrinology, Laboratory for General Clinical Chemistry, Paediatric Metabolic Diseases, APH - Personalized Medicine, ACS - Heart failure & arrhythmias |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty 030209 endocrinology & metabolism Critical Care and Intensive Care Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Oxidation medicine Humans Isotope ratio mass spectrometry Medium-chain triglyceride Triglycerides chemistry.chemical_classification Meal Carbon Isotopes 030109 nutrition & dietetics Nutrition and Dietetics business.industry Stable isotope ratio Fatty acid chain-elongation Fatty Acids Lipid kinetics Fatty acid Medium-chain fatty acids Carbon Dioxide Diet Enzyme Endocrinology chemistry Breath Tests Healthy individuals Isotope Labeling Caprylates Energy source business Oxidation-Reduction Blinded study |
| Zdroj: | Clinical nutrition (Edinburgh, Scotland), 40(3), 1396-1404. Churchill Livingstone |
| ISSN: | 0261-5614 |
| Popis: | Background & aims: Medium chain triglyceride (MCT) supplementation is often recommended as treatment for patients with long-chain fatty acid β-oxidation (lcFAO) disorders, since they can be utilized as an energy source without the use of the defective enzyme. However, studies in mice and preterm infants suggest that not all medium-chain fatty acids (MCFA) are oxidized and may undergo elongation to long-chain fatty acids (LCFA). In this single blinded study, we explored the metabolic fates of MCT in healthy individuals using a 13C-labeled MCT tracer. Method: Three healthy males in rest received on two test days a primed continuous infusion of glyceryl tri[1,2,3,4–13C4]-octanoate with either an isocaloric supplementation of 1) exclusively MCT (MCT-only) or 2) a mixture of MCT, proteins and carbohydrates (MCT-mix). Gas chromatography - combustion - isotope ratio mass spectrometry (GC-C-IRMS) was used to determine 13C-enrichment of long-chain fatty acids in plasma and of 13CO2 in exhaled air. Results: When provided as single energy source, an estimated 42% of administered MCT was converted to CO2. In combination with carbohydrates and proteins in the diet, oxidation of MCT was higher (62%). In both diets |
| Databáze: | OpenAIRE |
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