CD4+ T Cell Epitopes of FliC Conserved between Strains of Burkholderia: Implications for Vaccines against Melioidosis and Cepacia Complex in Cystic Fibrosis
| Autor: | Darawan Rinchai, Karen K. Y. Chu, Prasong Khaenam, Arnone Nithichanon, Ganjana Lertmemongkolchai, Anthony De Soyza, Catherine J. Reynolds, Emmanuel Favry, Julie A. Musson, Natasha Spink, Rosemary J. Boyton, John H. Robinson, Bernard Maillere, Daniel M. Altmann, Gregory J. Bancroft |
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| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
Burkholderia pseudomallei Melioidosis Cystic Fibrosis Burkholderia cenocepacia Burkholderia Immunology Epitopes T-Lymphocyte Mice Transgenic T-Cell Antigen Receptor Specificity Peptide binding Human leukocyte antigen Cross Reactions Biology Article Epitope Microbiology Interferon-gamma Mice Bacterial Proteins medicine Animals Humans Immunology and Allergy Alleles Burkholderia multivorans Histocompatibility Antigens Class II Burkholderia Infections HLA-DR Antigens bacterial infections and mycoses medicine.disease biology.organism_classification Virology Bacterial Vaccines bacteria Immunization Peptides Protein Binding |
| Zdroj: | The Journal of Immunology. 193:6041-6049 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Burkholderia pseudomallei is the causative agent of melioidosis characterized by pneumonia and fatal septicemia and prevalent in Southeast Asia. Related Burkholderia species are strong risk factors of mortality in cystic fibrosis (CF). The B. pseudomallei flagellar protein FliC is strongly seroreactive and vaccination protects challenged mice. We assessed B. pseudomallei FliC peptide binding affinity to multiple HLA class II alleles and then assessed CD4 T cell immunity in HLA class II transgenic mice and in seropositive individuals in Thailand. T cell hybridomas were generated to investigate cross-reactivity between B. pseudomallei and the related Burkholderia species associated with Cepacia Complex CF. B. pseudomallei FliC contained several peptide sequences with ability to bind multiple HLA class II alleles. Several peptides were shown to encompass strong CD4 T cell epitopes in B. pseudomallei–exposed individuals and in HLA transgenic mice. In particular, the p38 epitope is robustly recognized by CD4 T cells of seropositive donors across diverse HLA haplotypes. T cell hybridomas against an immunogenic B. pseudomallei FliC epitope also cross-reacted with orthologous FliC sequences from Burkholderia multivorans and Burkholderia cenocepacia, important pathogens in CF. Epitopes within FliC were accessible for processing and presentation from live or heat-killed bacteria, demonstrating that flagellin enters the HLA class II Ag presentation pathway during infection of macrophages with B. cenocepacia. Collectively, the data support the possibility of incorporating FliC T cell epitopes into vaccination programs targeting both at-risk individuals in B. pseudomallei endemic regions as well as CF patients. |
| Databáze: | OpenAIRE |
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