microRNA-122 abundance in hepatocellular carcinoma and non-tumor liver tissue from Japanese patients with persistent HCV versus HBV infection
| Autor: | Shuichi Kaneko, Robert E. Lanford, Masao Honda, Daisuke Yamane, Tetsuro Shimakami, Carolyn Spaniel, Stanley M. Lemon, Sara R. Selitsky |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Carcinoma Hepatocellular Pan troglodytes Hepatitis C virus lcsh:Medicine Biology medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Asian People Interferon medicine Animals Humans lcsh:Science Aged 030304 developmental biology Hepatitis B virus 0303 health sciences Multidisciplinary Liver Neoplasms lcsh:R Cancer virus diseases Hepatitis C Middle Aged Hepatitis B medicine.disease digestive system diseases 3. Good health Gene Expression Regulation Neoplastic MicroRNAs Liver 030220 oncology & carcinogenesis Hepatocellular carcinoma Immunology Female lcsh:Q Interferons Liver cancer Research Article medicine.drug |
| Zdroj: | PLoS ONE, Vol 8, Iss 10, p e76867 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Mechanisms of hepatic carcinogenesis in chronic hepatitis B and hepatitis C are incompletely defined but often assumed to be similar and related to immune-mediated inflammation. Despite this, several studies hint at differences in expression of miR-122, a liver-specific microRNA with tumor suppressor properties, in hepatocellular carcinoma (HCC) associated with hepatitis B virus (HBV) versus hepatitis C virus (HCV) infection. Differences in the expression of miR-122 in these cancers would be of interest, as miR-122 is an essential host factor for HCV but not HBV replication. To determine whether the abundance of miR-122 in cancer tissue is influenced by the nature of the underlying virus infection, we measured miR-122 by qRT-PCR in paired tumor and non-tumor tissues from cohorts of HBV- and HCV-infected Japanese patients. miR-122 abundance was significantly reduced from normal in HBV-associated HCC, but not in liver cancer associated with HCV infection. This difference was independent of the degree of differentiation of the liver cancer. Surprisingly, we also found significant differences in miR-122 expression in non-tumor tissue, with miR-122 abundance reduced from normal in HCV- but not HBV-infected liver. Similar differences were observed in HCV- vs. HBV-infected chimpanzees. Among HCV-infected Japanese subjects, reductions in miR-122 abundance in non-tumor tissue were associated with a single nucleotide polymorphism near the IL28B gene that predicts poor response to interferon-based therapy (TG vs. TT genotype at rs8099917), and correlated negatively with the abundance of multiple interferon-stimulated gene transcripts. Reduced levels of miR-122 in chronic hepatitis C thus appear to be associated with endogenous interferon responses to the virus, while differences in miR-122 expression in HCV- versus HBV-associated HCC likely reflect virus-specific mechanisms contributing to carcinogenesis. The continued expression of miR-122 in HCV-associated HCC may signify an important role for HCV replication late in the progression to malignancy. |
| Databáze: | OpenAIRE |
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