Amiloride fluxes across erythrocyte membranes
| Autor: | Juan G. Reyes, Dale J. Benos, David G. Shoemaker |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Chromatography
Cell Membrane Permeability Sodium Erythrocyte Membrane Biophysics chemistry.chemical_element Cell Biology Biochemistry Amiloride Red blood cell Membrane medicine.anatomical_structure chemistry Permeability (electromagnetism) Pyrazines Cultured cell medicine Humans Intracellular Ion transporter Mathematics medicine.drug |
| Zdroj: | Biochimica et biophysica acta. 734(1) |
| ISSN: | 0006-3002 |
| Popis: | Amiloride is known to inhibit both the influx of Na+ and the activation of mitogenesis in many cultured cell lines. This paper describes experiments in which the permeability coefficient of amiloride was determined from measurements of tracer fluxes across human erythrocytes and resealed ghosts. From an analysis of these fluxes, a permeability coefficient of 10(-7) cm/s for the uncharged form of amiloride was deduced. Based upon this measured permeability value, we present calculations of intracellular accumulation times of amiloride in cells of differing surface-to-volume ratio. |
| Databáze: | OpenAIRE |
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