Peptide 1018 inhibits swarming and influences Anr-regulated gene expression downstream of the stringent stress response in Pseudomonas aeruginosa

Autor: Bing C Wu, Amy S. Lee, Robert E. W. Hancock, Daniel Pletzer, Morgan A. Alford, Shannon R. Coleman, Reza Falsafi, Lauren Wilkinson, Travis M. Blimkie, Manjeet Bains
Rok vydání: 2021
Předmět:
Mutant
Swarming (honey bee)
Gene Expression
Swarming motility
Pathology and Laboratory Medicine
medicine.disease_cause
Mobile Genetic Elements
Gene expression
Medicine and Health Sciences
Cellular Stress Responses
0303 health sciences
Multidisciplinary
Virulence
Pseudomonas Aeruginosa
Drug Resistance
Microbial
Genomics
Bacterial Pathogens
Human morbidity
Cell Processes
Medical Microbiology
Medicine
Pathogens
Research Article
Pathogen Motility
Virulence Factors
Science
Motility
Library Screening
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Genetic Elements
Bacterial Proteins
Gene Types
Pseudomonas
Genetics
medicine
Humans
Pseudomonas Infections
Gene Regulation
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
030304 developmental biology
Molecular Biology Assays and Analysis Techniques
Bacteria
030306 microbiology
Pseudomonas aeruginosa
Transposable Elements
Organisms
Biology and Life Sciences
Cell Biology
biochemical phenomena
metabolism
and nutrition
Biofilms
Trans-Activators
Regulator Genes
Peptides
Zdroj: PLoS ONE, Vol 16, Iss 4, p e0250977 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Pseudomonas aeruginosais a ubiquitous opportunistic pathogen that causes considerable human morbidity and mortality, particularly in nosocomial infections and individuals with cystic fibrosis.P.aeruginosacan adapt to surface growth by undergoing swarming motility, a rapid multicellular movement that occurs on viscous soft surfaces with amino acids as a nitrogen source. Here we tested the small synthetic host defense peptide, innate defense regulator 1018, and found that it inhibited swarming motility at concentrations as low as 0.75 μg/ml, well below the MIC for strain PA14 planktonic cells (64 μg/ml). A screen of the PA14 transposon insertion mutant library revealed 29 mutants that were more tolerant to peptide 1018 during swarming, five of which demonstrated significantly greater swarming than the WT in the presence of peptide. Transcriptional analysis (RNA-Seq) of cells that were inoculated on swarming plates containing 1.0 μg/ml peptide revealed differential expression of 1,190 genes compared to cells swarming on plates without peptide. Furthermore, 1018 treatment distinctly altered the gene expression profile of cells when compared to that untreated cells in the centre of the swarm colonies. Peptide-treated cells exhibited changes in the expression of genes implicated in the stringent stress response including those regulated byanr, which is involved in anaerobic adaptation, indicative of a mechanism by which 1018 might inhibit swarming motility. Overall, this study illustrates potential mechanisms by which peptide 1018 inhibits swarming surface motility, an important bacterial adaptation associated with antibiotic resistance, virulence, and dissemination ofP.aeruginosa.
Databáze: OpenAIRE
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