Monocytes undergo multi-step differentiation in mice during oral infection by Toxoplasma gondii
| Autor: | Aurélie Detavernier, Laurye Van Maele, Emilie Calonne, Marion Splittgerber, François Fuks, Hussein Shehade, Muriel Nguyen, Stanislas Goriely, David Svec, Séverine Thomas, Abdulkader Azouz, Younes Achouri, Guillaume Oldenhove |
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| Přispěvatelé: | UCL - SSS/DDUV - Institut de Duve, UCL - SSS/DDUV/LPAD - Liver and pancreas differentiation |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Medicine (miscellaneous) Spleen Article Monocytes General Biochemistry Genetics and Molecular Biology Epigenesis Genetic Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine Animals lcsh:QH301-705.5 Monocytes and macrophages Lamina propria biology Interleukins Gene Expression Profiling Macrophages Computational Biology Toxoplasma gondii Interleukin Généralités Cell Differentiation Epigenetics in immune cells Cellular Reprogramming biology.organism_classification 030104 developmental biology IRF1 medicine.anatomical_structure lcsh:Biology (General) Monocyte differentiation Immunology Bone marrow Single-Cell Analysis Infection General Agricultural and Biological Sciences Toxoplasma Toxoplasmosis 030215 immunology |
| Zdroj: | Communications Biology Communications biology, Vol. 2, no.1, p. 472 [1-14] (2019) Communications Biology, Vol 2, Iss 1, Pp 1-14 (2019) Communications Biology, 2 (1 |
| ISSN: | 2399-3642 |
| Popis: | Monocytes play a major role in the defense against pathogens. They are rapidly mobilized to inflamed sites where they exert both proinflammatory and regulatory effector functions. It is still poorly understood how this dynamic and exceptionally plastic system is controlled at the molecular level. Herein, we evaluated the differentiation process that occurs in Ly6Chi monocytes during oral infection by Toxoplasma gondii. Flow cytometry and single-cell analysis revealed distinct activation status and gene expression profiles in the bone marrow, the spleen and the lamina propria of infected mice. We provide further evidence that acquisition of effector functions, such as the capacity to produce interleukin-27, is accompanied by distinct waves of epigenetic programming, highlighting a role for STAT1/IRF1 in the bone marrow and AP-1/NF-κB in the periphery. This work broadens our understanding of the molecular events that occur in vivo during monocyte differentiation in response to inflammatory cues. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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