Fusion of monocytes and macrophages with HIV-1 correlates with biochemical properties of CXCR4 and CCR5
| Autor: | Hana Golding, Cheryl K. Lapham, Tatiana Romanstseva, Shirley Lee, Marina Zaitseva |
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| Rok vydání: | 1999 |
| Předmět: |
Cell type
Receptors CXCR4 Receptors CCR5 Genetic Vectors Human immunodeficiency virus (HIV) Vaccinia virus Biology medicine.disease_cause CXCR4 General Biochemistry Genetics and Molecular Biology Monocytes Cell Fusion Western blot medicine Animals Humans Receptor Cell fusion U937 cell medicine.diagnostic_test Macrophages virus diseases General Medicine Precipitin Molecular biology Precipitin Tests CD4 Antigens HIV-1 Rabbits |
| Zdroj: | Nature medicine. 5(3) |
| ISSN: | 1078-8956 |
| Popis: | Human macrophages can be infected more efficiently by M-tropic than by T-tropic HIV-1 strains, despite surface expression of both CXCR4 and CCR5 co-receptors. Western blot analyses of total cell extracts and surface proteins from multiple sets of monocytes and macrophages demonstrated substantial differences between CXCR4 molecules. CXCR4 was mainly a monomer in monocytes, but was mainly a species of higher molecular weight (90 kDa) on the surface of macrophages. CCR5 was monomeric in both cell types. A constitutive association between CD4 and the co-receptors was seen in monocytes and macrophages. However, CD4 co-precipitated with CCR5 and CXCR4 monomers, but not with the high-molecular-weight forms of CXCR4, indicating that the high-molecular-weight CXCR4 species in macrophages are not available for association with CD4, which may contribute to the inefficient entry of T-tropic strains into mature macrophages. |
| Databáze: | OpenAIRE |
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