Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
| Autor: | John G. Flanagan, Toshiaki Shigeoka, Lucia C. S. Wunderlich, Max Koppers, William A. Harris, Christine E. Holt, Maximilian Ah Jakobs, Clemens F. Kaminski, Julie Qiaojin Lin, Asha Dwivedy, Michael S Minett, Anaïs Bellon, Sixian Zhao, Pedro Vallejo-Ramirez, Roberta Cagnetta |
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| Přispěvatelé: | Koppers, Max [0000-0002-7751-1082], Wunderlich, Lucia Cs [0000-0001-7200-1713], Vallejo-Ramirez, Pedro [0000-0002-7879-6761], Qiaojin Lin, Julie [0000-0002-2669-6478], Jakobs, Maximilian Ah [0000-0002-0879-7937], Kaminski, Clemens F [0000-0002-5194-0962], Harris, William A [0000-0002-9995-8096], Holt, Christine E [0000-0003-2829-121X], Apollo - University of Cambridge Repository, Wunderlich, Lucia CS [0000-0001-7200-1713], Jakobs, Maximilian AH [0000-0002-0879-7937] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Retinal Ganglion Cells Proteome Xenopus RNA-binding protein Stimulation Interactome Ribosome Xenopus laevis 0302 clinical medicine retinal ganglion cell Biology (General) Receptor axon biology Chemistry General Neuroscience RNA-Binding Proteins Translation (biology) General Medicine Cell biology medicine.anatomical_structure Retinal ganglion cell Medicine Research Article Human Signal Transduction QH301-705.5 mRNA Science Receptors Cell Surface General Biochemistry Genetics and Molecular Biology guidance receptor 03 medical and health sciences medicine Animals RNA Messenger local protein synthesis General Immunology and Microbiology biology.organism_classification Axons 030104 developmental biology Protein Biosynthesis Ribosomes 030217 neurology & neurosurgery Developmental Biology Neuroscience |
| Zdroj: | eLife, Vol 8 (2019) eLife |
| Popis: | Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors associate with distinct sets of mRNAs and RNA-binding proteins. Cue stimulation of growing Xenopus retinal ganglion cell axons induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by co-exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and suggest a generalizable model for cue-selective control of the local proteome. |
| Databáze: | OpenAIRE |
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