Buccal micronucleus cytome assay: Inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil
| Autor: | Cesar Koppe Grisolia, Veronica Elisa Pimenta Vicentini, Igor Vivian de Almeida, Márcia Fernanda Correia Jardim Paz, Catarina Satie Takahashi, Sabrina Fuziger Inácio Brandão, Magaly Sales Monteiro, Nelson Jorge Carvalho Batista, Luiza Barbosa de Oliveira, Paula Rohr, Raquel Alves dos Santos, Juliana da Silva, Carmen Lucia Bassi Branco, Reynaldo Assis de Vasconcelos Lopes, Glenda Nicioli da Silva, Marcos Felipe de Oliveira Galvão, Érica de Melo Reis, Tathyana B. Piau, Mirian Oliveira Goulart, Silvia Regina Batistuzzo de Medeiros, Gabrieli Flesch da Silva |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Smoking habit Scoring criteria Biology Toxicology 03 medical and health sciences Young Adult 0302 clinical medicine Environmental health Biomonitoring Humans Inter-laboratory Micronuclei Chromosome-Defective Cell Nucleus Micronucleus Tests Cell Death Mouth Mucosa Epithelial Cells General Medicine Middle Aged 030104 developmental biology Healthy individuals Biomarker (medicine) Biological Assay Female Micronucleus Laboratories 030217 neurology & neurosurgery Brazil DNA Damage |
| Zdroj: | Toxicology letters. 333 |
| ISSN: | 1879-3169 |
| Popis: | The Buccal Micronucleus Cytome Assay (BMCyt) has become an important biomonitoring tool for assessing cytogenetic damage in many studied populations. Each laboratory applies protocols that vary according to the method of collecting and preparing samples. Besides, Brazil is a country of great territorial extensions that received immigrants from various parts of the world with different genetic backgrounds. Therefore, the present study aimed to evaluate the inter-laboratory variation in scoring the same set of slides using the more comprehensive scoring criteria, to standardize the BMCyt protocol, to observe the basal alterations in populations of different Brazilian regions and to compare it with other places around the world. Our results showed that a valuable number of laboratories participated, ten laboratories from different regions of the country, for the validation of the BMCyt in human biomonitoring studies, resulting in the 804 healthy individuals. This was possible because we observed: a range of measures needs to be considered, such as the baseline frequency of DNA damage and cell death in non-exposed individuals; age when grouped showed an influence on DNA damage, although when evaluated by group we did not see an influence; association between smoking habit and all endpoints of the BMCyt (except karyolytic cells) was evident; the basal MN frequency, in the majority of groups, follows those around the world; and the BMCyt was confirmed as a good health status biomarker. We emphasize the need for constant discussions on the parameters of cell death due to greater difficulty among the analyzers. |
| Databáze: | OpenAIRE |
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